Pretreatment effects on the hydrolysis of sago starch granules by raw starch-digesting amylase from Penicillium brunneum


Citation

Haska N., . and Ohta Y., . (1991) Pretreatment effects on the hydrolysis of sago starch granules by raw starch-digesting amylase from Penicillium brunneum. [Proceedings Paper]

Abstract

Several raw starch-digesting amylases are produced from microorganisms including those isolated from Penicillium brunneum from sago palm. All these enzymes were found to be effective for hydrolysing cereal starches but root starches and sago starch were resistant to their action. Pretreatment of sago starch by heating it to a temperature below gelatinization temperature at lower pHs resulted in an increase in the ability of enzyme to digest sago starch. Heating to 65 percent centigrade at pH 3.5 resulted in a 86 percent conversion of sago starch to glucose compared with a 97 percent conversion of raw corn starch. Treatment of sago starch at 60 degrees centigrade and pH 3.5 increased the degree of hydrolysis to 200 percent compared to untreated sago starch the degree of hydrolysis of which is only 31 percent after 48 hr of incubation. Addition of the enzyme in small portion at various time intervals was found to be more effective in hydrolysing starch 91 percent than adding the total quantity in one bulk 86 percent


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Abstract

Several raw starch-digesting amylases are produced from microorganisms including those isolated from Penicillium brunneum from sago palm. All these enzymes were found to be effective for hydrolysing cereal starches but root starches and sago starch were resistant to their action. Pretreatment of sago starch by heating it to a temperature below gelatinization temperature at lower pHs resulted in an increase in the ability of enzyme to digest sago starch. Heating to 65 percent centigrade at pH 3.5 resulted in a 86 percent conversion of sago starch to glucose compared with a 97 percent conversion of raw corn starch. Treatment of sago starch at 60 degrees centigrade and pH 3.5 increased the degree of hydrolysis to 200 percent compared to untreated sago starch the degree of hydrolysis of which is only 31 percent after 48 hr of incubation. Addition of the enzyme in small portion at various time intervals was found to be more effective in hydrolysing starch 91 percent than adding the total quantity in one bulk 86 percent

Additional Metadata

[error in script]
Item Type: Proceedings Paper
Additional Information: 2 tables; 8 figs.; 19 refs.; Summary En
AGROVOC Term: SAGU
AGROVOC Term: ALMIDON
AGROVOC Term: HIDROLISIS
AGROVOC Term: PENICILLIUM
AGROVOC Term: AMILOSA/ MICROORGANISMOS
Geographical Term: MALAYSIA
Depositing User: Ms. Norfaezah Khomsan
Last Modified: 24 Apr 2025 05:26
URI: http://webagris.upm.edu.my/id/eprint/15022

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