The effect of temperature and time processing on the isoflavone content from fermented soybean


Citation

Harisun Yaakob, . and Noor Azwani Zainol, . and Ramlan Abd. Aziz, . and Mohamad Roji Sarmidi, . (2004) The effect of temperature and time processing on the isoflavone content from fermented soybean. [Proceedings Paper]

Abstract

Isoflavone is a natural phytochemical content in soy bean. There are four active ingredients in isoflavone such as genestein genestin daidzein and daidzin. The objective of this study was to evaluate the effects of the parameter such as temperature and time of fermentation on the content of isoflavone. Saponification method with ethanol/water mixture was used as a solvent in the isoflavone extraction process. The isoflavone content determined by optical density method using a spectrophotometer. Optical density was detected at 263nm for gene stein and genestin and 249nm detected for daidzein and daidzin. The time and temperature of fermentation influence the percentage of isoflavone content. The temperature at 35C and the fermentation time of 48 result in a high yield of isoflavone. The highest amount of diadzein diadzin gene stein and genestin are 1.23mug/g 0.54mug/g 0.5mug/g and 0.27mug/g.


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Abstract

Isoflavone is a natural phytochemical content in soy bean. There are four active ingredients in isoflavone such as genestein genestin daidzein and daidzin. The objective of this study was to evaluate the effects of the parameter such as temperature and time of fermentation on the content of isoflavone. Saponification method with ethanol/water mixture was used as a solvent in the isoflavone extraction process. The isoflavone content determined by optical density method using a spectrophotometer. Optical density was detected at 263nm for gene stein and genestin and 249nm detected for daidzein and daidzin. The time and temperature of fermentation influence the percentage of isoflavone content. The temperature at 35C and the fermentation time of 48 result in a high yield of isoflavone. The highest amount of diadzein diadzin gene stein and genestin are 1.23mug/g 0.54mug/g 0.5mug/g and 0.27mug/g.

Additional Metadata

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Item Type: Proceedings Paper
Additional Information: 2 tables
AGROVOC Term: FLAVONOIDS
AGROVOC Term: FERMENTED FOODS
AGROVOC Term: SOYBEANS
AGROVOC Term: FERMENTATION
AGROVOC Term: TEMPERATURE
AGROVOC Term: SAPONIFICATION
AGROVOC Term: SOLVENT EXTRACTION
AGROVOC Term: YIELDS
AGROVOC Term: MALAYSIA
Geographical Term: MALAYSIA
Depositing User: Ms. Norfaezah Khomsan
Last Modified: 24 Apr 2025 05:26
URI: http://webagris.upm.edu.my/id/eprint/15606

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