Effects of different precursors on the biomass and triterpenoid production in callus cultures of Centella asiatica

Marziah M., . and Ling Anna P.K., . (2002) Effects of different precursors on the biomass and triterpenoid production in callus cultures of Centella asiatica. [Proceedings Paper]

Centella asiatica is a slender tropical herbaceous plant with crawling stems propagated vegetatively by stolons. It has become popular because of its pharmaceutical and cosmetic properties owing mainly to the presence of secondary metabolites called triterpenes. Many approaches have been considered to increase the production of secondary metabolites in plant tissue cultures one of which is through the addition of elicitor and precursors into the tissue culture media. The successful results obtained using this approach have been reported in many previous studies. The objective of this study is to determine the effects of different concentrations of precursors on biomass as well as the triterpene production in Centella asiatica. Squalene farnesyl pyrophosphate FPP isopentyl pyrophosphate IPP and leucine which are known to involve directly or indirectly in the triterpene biosynthetic pathway were used in this study. Based on the data obtained a low concentration of squalene 0.08 mg/l was found to be the best precursor for biomass production and the highest enhancement of the targeted triterpene production was observed at 0.4 mg/l squalene.

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Abstract

Centella asiatica is a slender tropical herbaceous plant with crawling stems propagated vegetatively by stolons. It has become popular because of its pharmaceutical and cosmetic properties owing mainly to the presence of secondary metabolites called triterpenes. Many approaches have been considered to increase the production of secondary metabolites in plant tissue cultures one of which is through the addition of elicitor and precursors into the tissue culture media. The successful results obtained using this approach have been reported in many previous studies. The objective of this study is to determine the effects of different concentrations of precursors on biomass as well as the triterpene production in Centella asiatica. Squalene farnesyl pyrophosphate FPP isopentyl pyrophosphate IPP and leucine which are known to involve directly or indirectly in the triterpene biosynthetic pathway were used in this study. Based on the data obtained a low concentration of squalene 0.08 mg/l was found to be the best precursor for biomass production and the highest enhancement of the targeted triterpene production was observed at 0.4 mg/l squalene.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. mal QK99 A1S471 2001 Call Number.
AGROVOC Term:	HERBACEOUS PLANTS
AGROVOC Term:	TRITERPENOIDS
AGROVOC Term:	BIOMASS
AGROVOC Term:	PLANT TISSUES
AGROVOC Term:	TISSUE CULTURE
AGROVOC Term:	CALLUS
AGROVOC Term:	MALAYSIA
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:26
URI:	http://webagris.upm.edu.my/id/eprint/15626

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