Regulation of oil palm Elaeis guineensis zeaxanthin epoxidase in developing mesocarp tissues determined by real-time PCR

Rasid O.A., . and Nor Hanin A., . and Masura S.S., . and Ho C.L., . and Suhaimi N., . and Sambanthamurthi R., . (2007) Regulation of oil palm Elaeis guineensis zeaxanthin epoxidase in developing mesocarp tissues determined by real-time PCR. [Proceedings Paper]

The potential health benefits of carotenoids as anticancer and antioxidant agents have recently been demonstrated. Oil palm is known to be the richest natural source for carotene and thus has great advantages for future genetic engineering work. However the knowledge in the oil palm carotenoid biosynthetic pathway especially on the regulation of its genes and the pathway itself is still quite limited. The current work describes the expression pattern of oil palm zeaxanthin eposidase zep the enzyme that catalyzes the formation of violaxanthin in developing fruits by real-time PCR analysis. Results indicated that the gene transcript was steadily increased from 5 WAA week after anthesis to 13 WAA tissues at about 1.19 fold. The increase may indicate the requirement for carotenoids or abscisic acid ABA for the development. Then the transcript decreased in the 15 through 17 WAA tissues coincide with the onset of oil synthesis in the oil palm fruit. Interestingly the level was shown to be increased again in the 19 WAA tissues. The expression of the gene is comparatively higher in young and matured leaves than in the mesocarp tissues at 1.83 fold and 2.87 fold respectively. This result may reflect the bigger requirement of oxygenated carotenoids in the photosynthetically active tissues. In conclusion the results from this study demonstrated that oil palm zep was highly regulated for tissue development and carotenoid requirement for the photosynthetic process. Thus it is very likely that post transcriptional level could be an important regulatory mechanism for carotenoid synthesis and accumulation in oil palm fruits and leaves.

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Abstract

The potential health benefits of carotenoids as anticancer and antioxidant agents have recently been demonstrated. Oil palm is known to be the richest natural source for carotene and thus has great advantages for future genetic engineering work. However the knowledge in the oil palm carotenoid biosynthetic pathway especially on the regulation of its genes and the pathway itself is still quite limited. The current work describes the expression pattern of oil palm zeaxanthin eposidase zep the enzyme that catalyzes the formation of violaxanthin in developing fruits by real-time PCR analysis. Results indicated that the gene transcript was steadily increased from 5 WAA week after anthesis to 13 WAA tissues at about 1.19 fold. The increase may indicate the requirement for carotenoids or abscisic acid ABA for the development. Then the transcript decreased in the 15 through 17 WAA tissues coincide with the onset of oil synthesis in the oil palm fruit. Interestingly the level was shown to be increased again in the 19 WAA tissues. The expression of the gene is comparatively higher in young and matured leaves than in the mesocarp tissues at 1.83 fold and 2.87 fold respectively. This result may reflect the bigger requirement of oxygenated carotenoids in the photosynthetically active tissues. In conclusion the results from this study demonstrated that oil palm zep was highly regulated for tissue development and carotenoid requirement for the photosynthetic process. Thus it is very likely that post transcriptional level could be an important regulatory mechanism for carotenoid synthesis and accumulation in oil palm fruits and leaves.

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Item Type:	Proceedings Paper
AGROVOC Term:	ELAEIS GUINEENSIS
AGROVOC Term:	OIL PALMS
AGROVOC Term:	XANTHOPHYLLS
AGROVOC Term:	EPOXY COMPOUNDS
AGROVOC Term:	PERICARP
AGROVOC Term:	PCR
AGROVOC Term:	CAROTENOIDS
AGROVOC Term:	MALAYSIA
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:27
URI:	http://webagris.upm.edu.my/id/eprint/15718

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