Isolation and identification of Helicobacter pullorum from broiler chickens in Malaysia

Soe Soe Wai, . and Saleha A. A., . and Zunita Z., . and Hassan I., . and Jalila A., . (2009) Isolation and identification of Helicobacter pullorum from broiler chickens in Malaysia. [Proceedings Paper]

Helicobacter pullorum is increasingly recognized as a foodborne pathogen in humans. A number of metho1s have been described for the detection of H. pullorum in chickens. This study was carried out to investigate the occurrence of H. pullorum in broiler chickens. A total of 50 live chickens were purchased from five farms then slaughtered and their caeca were collected. The caecal faeces were obtained aseptically from the caeca of each chicken and subjected to a modified membrane filter method as described by Miller et aI. 2006 method M and a method which was a further modification to method M method S. Biochemical tests were done on the suspected Helicobacter isolates and were confirmed using modified species-specific PCR. Thirty seven out of 50 74 chickens were positive for H.pullorum using method S while 22 44 positive using method M. The isolation method modified in this study gave a better recovery of H. pullorum from chicken caecal contents. This study indicates that broiler chickens may serve as reservoirs of H. pullorum and represent a health risk to humans.

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Abstract

Helicobacter pullorum is increasingly recognized as a foodborne pathogen in humans. A number of metho1s have been described for the detection of H. pullorum in chickens. This study was carried out to investigate the occurrence of H. pullorum in broiler chickens. A total of 50 live chickens were purchased from five farms then slaughtered and their caeca were collected. The caecal faeces were obtained aseptically from the caeca of each chicken and subjected to a modified membrane filter method as described by Miller et aI. 2006 method M and a method which was a further modification to method M method S. Biochemical tests were done on the suspected Helicobacter isolates and were confirmed using modified species-specific PCR. Thirty seven out of 50 74 chickens were positive for H.pullorum using method S while 22 44 positive using method M. The isolation method modified in this study gave a better recovery of H. pullorum from chicken caecal contents. This study indicates that broiler chickens may serve as reservoirs of H. pullorum and represent a health risk to humans.

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Item Type:	Proceedings Paper
Additional Information:	13 ref
AGROVOC Term:	Helicobacter
AGROVOC Term:	Broiler chickens
AGROVOC Term:	Isolation techniques
AGROVOC Term:	Foodborne diseases
AGROVOC Term:	Malaysia
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:27
URI:	http://webagris.upm.edu.my/id/eprint/15828

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