In vitro screening of local medicinal plants for antimalarial activity to malaria parasite Plasmodium falciparum

Noor Rain A., . and Sajiri S., . and Rohaya C., . and Normaznah Y., . (2000) In vitro screening of local medicinal plants for antimalarial activity to malaria parasite Plasmodium falciparum. [Proceedings Paper]

Among the strategies for the development of traditional medicine research in Malaysia is the discovery of natural products for new anti-malarial agents. Thirty-five methanolic crude extracts from local medicinal plants were screened for anti-malarial activities against a local isolate Gombak A Chloroquine resistant of the malaria parasite Plasmodium falciparum. The anti-malarial activities of the extracts were screened using two tests: Test A Inhibition of the development of ring and trophozoite stage to schizont maturationand Test B Inhibition of the invasion of merozoite stage into the red blood cells using the merozoite invasion assay. Both tests have basically similar procedures. Briefly 1 to 2 per cent of ring stage and trophozoite infected blood for Test A and schizont infected blood for Test B were exposed to a range of extract concentrations in duplicates across the 96-wells flat bottomed tissue culture plate. The extract concentrations were serially diluted two-fold in culture medium containing RPMI 1640 Roswell Park Memorial Institute medium 1640 25mM HEPES N-2-Hydroxyethyl piperazine-N'-2-ethane sulfonic acid and 32mM NaHCO subscript 3 supplemented with 10 per cent human serum. The plates were agitated to ensure suspension then placed in a candle jar at 37 degree Celcius for 24 hours for Test A and 48 hours for Test B respectively. Parasitemia and parasite stage were determined microscopically on thin blood films stained with Giemsa. The results were analysed statistically using Probit Analysis. The results showed that of the 35 plant extracts nine had schizonticidal activity while seven had inhibitory activity on the merozoite invasion into erythrocyte. The selection was based on the effective dose fifty ED subscript 50 values of the extracts in comparison with the ED subscript 50 value of chloroquine and the percentage inhibition of these extracts on the development of the parasite. The ED subscript 50 values for the extracts selected were found to be between 0.0 to 1.8 microgram/ml.

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Abstract

Among the strategies for the development of traditional medicine research in Malaysia is the discovery of natural products for new anti-malarial agents. Thirty-five methanolic crude extracts from local medicinal plants were screened for anti-malarial activities against a local isolate Gombak A Chloroquine resistant of the malaria parasite Plasmodium falciparum. The anti-malarial activities of the extracts were screened using two tests: Test A Inhibition of the development of ring and trophozoite stage to schizont maturationand Test B Inhibition of the invasion of merozoite stage into the red blood cells using the merozoite invasion assay. Both tests have basically similar procedures. Briefly 1 to 2 per cent of ring stage and trophozoite infected blood for Test A and schizont infected blood for Test B were exposed to a range of extract concentrations in duplicates across the 96-wells flat bottomed tissue culture plate. The extract concentrations were serially diluted two-fold in culture medium containing RPMI 1640 Roswell Park Memorial Institute medium 1640 25mM HEPES N-2-Hydroxyethyl piperazine-N'-2-ethane sulfonic acid and 32mM NaHCO subscript 3 supplemented with 10 per cent human serum. The plates were agitated to ensure suspension then placed in a candle jar at 37 degree Celcius for 24 hours for Test A and 48 hours for Test B respectively. Parasitemia and parasite stage were determined microscopically on thin blood films stained with Giemsa. The results were analysed statistically using Probit Analysis. The results showed that of the 35 plant extracts nine had schizonticidal activity while seven had inhibitory activity on the merozoite invasion into erythrocyte. The selection was based on the effective dose fifty ED subscript 50 values of the extracts in comparison with the ED subscript 50 value of chloroquine and the percentage inhibition of these extracts on the development of the parasite. The ED subscript 50 values for the extracts selected were found to be between 0.0 to 1.8 microgram/ml.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. RS164 S471 1999 Call Number
AGROVOC Term:	DRUG PLANTS
AGROVOC Term:	PLANT EXTRACTS
AGROVOC Term:	ANTIMICROBIAL PROPERTIES
AGROVOC Term:	MEDICINAL PROPERTIES
AGROVOC Term:	TRADITIONAL MEDICINES
AGROVOC Term:	IN VITRO EXPERIMENTATION
AGROVOC Term:	TESTING
AGROVOC Term:	PROTOZOAL INFECTIONS
AGROVOC Term:	MALARIA
AGROVOC Term:	PLASMODIUM FALCIPARUM
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:27
URI:	http://webagris.upm.edu.my/id/eprint/15890

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