Citation
Asmah Rahmat, . and Eng P.W., . and Susi Endrini, . (2001) Effect of wheatgrass Agropyron trachycalum on the proliferation of liver cancer cell lines HepG2. [Proceedings Paper]
Abstract
The consumption of cereal sprouts as a health food is gaining popularity in our community. Wheatgrass is as example of cereal sprouts which can be obtained easily from the local market. This study was designed to determine the effect of wheatgrass juice extract on the proliferation of liver cancer cell lices HepG2 in vitro at different incubation periods. Normal liver cell lines Chang Liver was used as a control to determine the selective cytotoxic of the treatment. Cell proliferation assay was done using MTT 3-4 5-dimethylthiazol-2-yl-2 5-diphenyl tetrazolium bromide assay method. The concentration that cause 50 percent cell death of HepG2 cell lines IC50 of each treatment were determined from the Graph of Percentage of Cytotoxicity Against log10 Concentration of Juice Extract Vs/Vm. From this study IC50 was obtained from the wheatgrass treatment against the HepG2 cell lines with 24 hours and 48 hours incubation period with the reading 2.3036 x 10-3 Vs/Vm31.89 microgram/ml and 1.7713 x 10-3 Vs/Vm. respectively. However there was no IC500 for 72 hours incubation period. IC50 was not obtained from the wheatgrass treatment against the Chang Liver cells. Fourty eight 48 hours incubation period for wheatgrass treatment is found to be more effective than other incubation period. Cytotoxicity of wheatgrass was found to be selective against HepG2 cells not to Chang Liver cells. This study concluded that the wheatgrass had the potential as the anticancer substance for liver cancer.
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Abstract
The consumption of cereal sprouts as a health food is gaining popularity in our community. Wheatgrass is as example of cereal sprouts which can be obtained easily from the local market. This study was designed to determine the effect of wheatgrass juice extract on the proliferation of liver cancer cell lices HepG2 in vitro at different incubation periods. Normal liver cell lines Chang Liver was used as a control to determine the selective cytotoxic of the treatment. Cell proliferation assay was done using MTT 3-4 5-dimethylthiazol-2-yl-2 5-diphenyl tetrazolium bromide assay method. The concentration that cause 50 percent cell death of HepG2 cell lines IC50 of each treatment were determined from the Graph of Percentage of Cytotoxicity Against log10 Concentration of Juice Extract Vs/Vm. From this study IC50 was obtained from the wheatgrass treatment against the HepG2 cell lines with 24 hours and 48 hours incubation period with the reading 2.3036 x 10-3 Vs/Vm31.89 microgram/ml and 1.7713 x 10-3 Vs/Vm. respectively. However there was no IC500 for 72 hours incubation period. IC50 was not obtained from the wheatgrass treatment against the Chang Liver cells. Fourty eight 48 hours incubation period for wheatgrass treatment is found to be more effective than other incubation period. Cytotoxicity of wheatgrass was found to be selective against HepG2 cells not to Chang Liver cells. This study concluded that the wheatgrass had the potential as the anticancer substance for liver cancer.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QD415 A1N277 2000 |
| AGROVOC Term: | AGROPYRON |
| AGROVOC Term: | LIVER DISEASES |
| AGROVOC Term: | CELLS |
| AGROVOC Term: | TISSUE PROLIFERATION |
| AGROVOC Term: | EXTRACTS |
| AGROVOC Term: | HEALTH FOODS |
| AGROVOC Term: | ANTIOXIDANTS |
| AGROVOC Term: | CULTIVATION |
| AGROVOC Term: | MALAYSIA |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Norfaezah Khomsan |
| Last Modified: | 24 Apr 2025 05:27 |
| URI: | http://webagris.upm.edu.my/id/eprint/15933 |
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