Citation
Harmin S.A., . and Kiong G.H., . and Ang K.J., . and Cheah S.H., . (1997) Ovulatory response in ketutu Oxyeleotris marmoratus following administration of human chorionic gonadotropin. [Proceedings Paper]
Abstract
Sexually maturing female ketutu Oxyeleotris marmoratus were injected with human chorionic gonadotropin HCG to determine the ovulatory response following administration of HCG. Fecundity fertilisation and hatching rates were determined in all the ovulated females. Females were checked for ovulation 12 hrs after the last injection. In experiment 1 a total of 45 females were utilised and they were randomly assigned into 15 groups n3 per group according to the different doses and days of hormone injections. Females in groups A-E F-J and K-O received a single injection of the hormone on the first day only injection on two consecutive days and injection on three consecutive days respectively. They were administrated with HCG at a single dose of 2 4 6 or 8 IU/g weight. Controls were given an equivalent volume of saline. About 67-100 of the female fish ovulated in the groups that received injection two and three days consecutively. There was no ovulatory response in all the females treated with saline. Fecundity fertilisation and hatching rate vary widely ranging from 150 to 2 300 eggs per 100 g body weight 5-42 and 5-27 respectively. In experiment 2 a similar protocol was used but the doses were partitioned into two injections 12 hrs apart. The results from experiment 2 indicated that split administration of the hormone did not give any advantage over the single administration in terms of ovulation fecundity 480-1 800 eggs/g body weight fertilisation 6-25 and hatching rates 2-18. No ovulatory response was observed in females receiving saline or 2 IU/g body weight. It appears that a single injection per day given on three consecutive days is a better protocol for inducing ovulation/spawning in ketutu when taking into consideration the cost of the hormone labour and the possible effect of stress due to frequent handling of fish during hormone therapy.
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Abstract
Sexually maturing female ketutu Oxyeleotris marmoratus were injected with human chorionic gonadotropin HCG to determine the ovulatory response following administration of HCG. Fecundity fertilisation and hatching rates were determined in all the ovulated females. Females were checked for ovulation 12 hrs after the last injection. In experiment 1 a total of 45 females were utilised and they were randomly assigned into 15 groups n3 per group according to the different doses and days of hormone injections. Females in groups A-E F-J and K-O received a single injection of the hormone on the first day only injection on two consecutive days and injection on three consecutive days respectively. They were administrated with HCG at a single dose of 2 4 6 or 8 IU/g weight. Controls were given an equivalent volume of saline. About 67-100 of the female fish ovulated in the groups that received injection two and three days consecutively. There was no ovulatory response in all the females treated with saline. Fecundity fertilisation and hatching rate vary widely ranging from 150 to 2 300 eggs per 100 g body weight 5-42 and 5-27 respectively. In experiment 2 a similar protocol was used but the doses were partitioned into two injections 12 hrs apart. The results from experiment 2 indicated that split administration of the hormone did not give any advantage over the single administration in terms of ovulation fecundity 480-1 800 eggs/g body weight fertilisation 6-25 and hatching rates 2-18. No ovulatory response was observed in females receiving saline or 2 IU/g body weight. It appears that a single injection per day given on three consecutive days is a better protocol for inducing ovulation/spawning in ketutu when taking into consideration the cost of the hormone labour and the possible effect of stress due to frequent handling of fish during hormone therapy.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| AGROVOC Term: | FISHES |
| AGROVOC Term: | OVULATION |
| AGROVOC Term: | FERTILIZATION |
| AGROVOC Term: | HATCHING |
| AGROVOC Term: | GONADOTROPINS |
| AGROVOC Term: | OVIPOSITION |
| AGROVOC Term: | HORMONES |
| AGROVOC Term: | THERAPY |
| AGROVOC Term: | MALAYSIA |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Norfaezah Khomsan |
| Last Modified: | 24 Apr 2025 05:27 |
| URI: | http://webagris.upm.edu.my/id/eprint/16129 |
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