Regeneration of Aerides multiflora roxb. using synthetic seeds: a study in-vitro

Vij S.P., . and Pathak P., . (2005) Regeneration of Aerides multiflora roxb. using synthetic seeds: a study in-vitro. [Proceedings Paper]

An imbalance between the efficiency of in-vitro propagation and the delivery of regenerants poses certain limitations on practical applications of tissue culture technology. It is in this context that the utility of synthetic seeds SyS as an efficient storage and delivery system is being increasingly realized. SyS are defined as artifically encapsuled somatic embryos shoot buds cell aggregates or any other tissue that can be used for sowing as seeds possess the ability to convert into a plant under in-vitro or ex vitro conditions and retain this potential also after storage. Ever since SyS were first prepared in carrots their production has been successfully extended to several flowering plant species including orchids. So far SyS have been produced in nearly 20 orchid species. Presently SyS were prepared for Aerides multiflora Roxb. a species of foxtail orchid with very attractive and long-lasting flowers arranged in long racemes which has been used to produce a variety of floriculturally significant hybrids. Uniform sized 0.1 cm protocorm-like bodies PLBs obtained from leaf cultures were used as encapsulants. The physical characteristics of the SyS size shape and texture varied with the concentration of gelling agents sodium alginate calcium chloride and the subsequent complexation period. SyS were best formed using a combination of 3.5 sodium alginate and 100 mM CaCI. 2HO with a complexation period of 30 minutes. A coating of talcum powder improved their quality as well as viability. The viability of the SyS declined gradually upon storage at ambient room temperature but when stored at low temperature 4 C the viability could be retained over longer periods. Amongst the various sowing substrata used agarised Mitra Prasad and Roychowdhury 1976M medium supported 100 conversion frequency. Encapsulated PLBs regenerated complete healthy plantlets with 2-3 leaves and 1-2 roots within 12 weeks. The regeneration of encapsulated PLBs even after storage of 120 days at 4C suggests that this technique can be effectively used for the preservation of commercially important and/or endangered orchid genotypes.

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Abstract

An imbalance between the efficiency of in-vitro propagation and the delivery of regenerants poses certain limitations on practical applications of tissue culture technology. It is in this context that the utility of synthetic seeds SyS as an efficient storage and delivery system is being increasingly realized. SyS are defined as artifically encapsuled somatic embryos shoot buds cell aggregates or any other tissue that can be used for sowing as seeds possess the ability to convert into a plant under in-vitro or ex vitro conditions and retain this potential also after storage. Ever since SyS were first prepared in carrots their production has been successfully extended to several flowering plant species including orchids. So far SyS have been produced in nearly 20 orchid species. Presently SyS were prepared for Aerides multiflora Roxb. a species of foxtail orchid with very attractive and long-lasting flowers arranged in long racemes which has been used to produce a variety of floriculturally significant hybrids. Uniform sized 0.1 cm protocorm-like bodies PLBs obtained from leaf cultures were used as encapsulants. The physical characteristics of the SyS size shape and texture varied with the concentration of gelling agents sodium alginate calcium chloride and the subsequent complexation period. SyS were best formed using a combination of 3.5 sodium alginate and 100 mM CaCI. 2HO with a complexation period of 30 minutes. A coating of talcum powder improved their quality as well as viability. The viability of the SyS declined gradually upon storage at ambient room temperature but when stored at low temperature 4 C the viability could be retained over longer periods. Amongst the various sowing substrata used agarised Mitra Prasad and Roychowdhury 1976M medium supported 100 conversion frequency. Encapsulated PLBs regenerated complete healthy plantlets with 2-3 leaves and 1-2 roots within 12 weeks. The regeneration of encapsulated PLBs even after storage of 120 days at 4C suggests that this technique can be effectively used for the preservation of commercially important and/or endangered orchid genotypes.

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Item Type:	Proceedings Paper
Additional Information:	3 ill 33 ref.
AGROVOC Term:	Orchidaceae
AGROVOC Term:	Regeneration
AGROVOC Term:	In vitro
AGROVOC Term:	Synthetic seeds
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:28
URI:	http://webagris.upm.edu.my/id/eprint/16638

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