Gamma irradiation to devitalize seeds of dried wheat and linseed flowers

Mohd Ridzuan Ismail, . and Siti Hajar Ahmad, . and U.R. Sinniah, . and Mohd Nazir Basiran, . and Phebe Ding, . (2003) Gamma irradiation to devitalize seeds of dried wheat and linseed flowers. [Proceedings Paper]

This study was carried out to obtain suitable irradiation dose to devitalize seeds of dried wheat Triticum sp. and linseed Linum sp. flowers. Seeds of dried wheat and linseed flowers were irradiated at doses of0 0.5 1 2 4 6 and 10 kGy. Irradiated seeds were tested for seed germination percentage viability and seedling vigor. Tetrazolium test was carried out to check for seed viability. Seedling vigor was determined by measuring shoot and root length on the 5th day of germination. Gamma irradiation did not inhibit seed germination of wheat and linseed dried flowers but delayed germination in wheat by three days when irradiated at 10 kGy. Soaking with 1 2 3 5-triphenyl-tetrazolium chloride solution showed that irradiated seeds were still viable as indicated by the red stained embryos and cotyledons. Shoot and root length of wheat and linseed from seedling growth test showed highly significant reduction even at low irradiation dose of 0.5 kGy. Except for seeds irradiated at 0.5 kGy the seedlings turned brownish and eventually died after 20 days. Roots were found to be more sensitive to irradiation whereby dose of 10 kGy inhibited root growth of wheat and linseed seedlings. The study showed that gamma irradiation of 1 kGy was sufficient to devitalize seeds of dried wheat and linseed flowers.

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Abstract

This study was carried out to obtain suitable irradiation dose to devitalize seeds of dried wheat Triticum sp. and linseed Linum sp. flowers. Seeds of dried wheat and linseed flowers were irradiated at doses of0 0.5 1 2 4 6 and 10 kGy. Irradiated seeds were tested for seed germination percentage viability and seedling vigor. Tetrazolium test was carried out to check for seed viability. Seedling vigor was determined by measuring shoot and root length on the 5th day of germination. Gamma irradiation did not inhibit seed germination of wheat and linseed dried flowers but delayed germination in wheat by three days when irradiated at 10 kGy. Soaking with 1 2 3 5-triphenyl-tetrazolium chloride solution showed that irradiated seeds were still viable as indicated by the red stained embryos and cotyledons. Shoot and root length of wheat and linseed from seedling growth test showed highly significant reduction even at low irradiation dose of 0.5 kGy. Except for seeds irradiated at 0.5 kGy the seedlings turned brownish and eventually died after 20 days. Roots were found to be more sensitive to irradiation whereby dose of 10 kGy inhibited root growth of wheat and linseed seedlings. The study showed that gamma irradiation of 1 kGy was sufficient to devitalize seeds of dried wheat and linseed flowers.

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Item Type:	Proceedings Paper
Additional Information:	Summary En
AGROVOC Term:	WHEATS
AGROVOC Term:	LINSEED
AGROVOC Term:	GAMMA IRRADIATION
AGROVOC Term:	DRYING
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:28
URI:	http://webagris.upm.edu.my/id/eprint/16910

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