Citation
Nor'aishah A.S., . and Radzali M., . (2003) Callus proliferation in woody plant of Michelia Alba dc. young flower and leaf explants. [Proceedings Paper]
Abstract
Two types of established basal media were used in order to select the best media for callus proliferation and organogenesis in Michelia alba flower and leaf explants. Petal excised from flower of stage 8 and lamina with midrib from leaf of stage 2 was separately cultured on MS 1962 medium and woody plant medium Lloyd and McCown 1981. Each of the studied media was supplemented with various concentrations of plant growth regulators such as NAA and BAP. Both media with supplemented with plant growth regulators have produced pale colour and friable callus. However the woody plant media produce remarkable callus proliferation compare to MS medium which are around 50 to 100 of the explants have induced callus formation. Most of the calluses were friable to compactly nodular and leaf explants produce yield than flower explants.
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Abstract
Two types of established basal media were used in order to select the best media for callus proliferation and organogenesis in Michelia alba flower and leaf explants. Petal excised from flower of stage 8 and lamina with midrib from leaf of stage 2 was separately cultured on MS 1962 medium and woody plant medium Lloyd and McCown 1981. Each of the studied media was supplemented with various concentrations of plant growth regulators such as NAA and BAP. Both media with supplemented with plant growth regulators have produced pale colour and friable callus. However the woody plant media produce remarkable callus proliferation compare to MS medium which are around 50 to 100 of the explants have induced callus formation. Most of the calluses were friable to compactly nodular and leaf explants produce yield than flower explants.
Additional Metadata
Item Type: | Proceedings Paper |
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Additional Information: | Received on 3 Dec 2007 QK710.2 M239 2003 |
AGROVOC Term: | CALLUS |
AGROVOC Term: | WOODY PLANTS |
AGROVOC Term: | MICHELIA |
AGROVOC Term: | BA |
AGROVOC Term: | NAA |
AGROVOC Term: | FLOWERS |
AGROVOC Term: | LEAVES |
Geographical Term: | MALAYSIA |
Depositing User: | Ms. Norfaezah Khomsan |
Last Modified: | 24 Apr 2025 05:28 |
URI: | http://webagris.upm.edu.my/id/eprint/16993 |
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