Callus proliferation in woody plant of Michelia Alba dc. young flower and leaf explants


Citation

Nor'aishah A.S., . and Radzali M., . (2003) Callus proliferation in woody plant of Michelia Alba dc. young flower and leaf explants. [Proceedings Paper]

Abstract

Two types of established basal media were used in order to select the best media for callus proliferation and organogenesis in Michelia alba flower and leaf explants. Petal excised from flower of stage 8 and lamina with midrib from leaf of stage 2 was separately cultured on MS 1962 medium and woody plant medium Lloyd and McCown 1981. Each of the studied media was supplemented with various concentrations of plant growth regulators such as NAA and BAP. Both media with supplemented with plant growth regulators have produced pale colour and friable callus. However the woody plant media produce remarkable callus proliferation compare to MS medium which are around 50 to 100 of the explants have induced callus formation. Most of the calluses were friable to compactly nodular and leaf explants produce yield than flower explants.


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Abstract

Two types of established basal media were used in order to select the best media for callus proliferation and organogenesis in Michelia alba flower and leaf explants. Petal excised from flower of stage 8 and lamina with midrib from leaf of stage 2 was separately cultured on MS 1962 medium and woody plant medium Lloyd and McCown 1981. Each of the studied media was supplemented with various concentrations of plant growth regulators such as NAA and BAP. Both media with supplemented with plant growth regulators have produced pale colour and friable callus. However the woody plant media produce remarkable callus proliferation compare to MS medium which are around 50 to 100 of the explants have induced callus formation. Most of the calluses were friable to compactly nodular and leaf explants produce yield than flower explants.

Additional Metadata

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Item Type: Proceedings Paper
Additional Information: Received on 3 Dec 2007 QK710.2 M239 2003
AGROVOC Term: CALLUS
AGROVOC Term: WOODY PLANTS
AGROVOC Term: MICHELIA
AGROVOC Term: BA
AGROVOC Term: NAA
AGROVOC Term: FLOWERS
AGROVOC Term: LEAVES
Geographical Term: MALAYSIA
Depositing User: Ms. Norfaezah Khomsan
Last Modified: 24 Apr 2025 05:28
URI: http://webagris.upm.edu.my/id/eprint/16993

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