The effect of different combinations of cytokinins on shoot proliferation of plant micropropagation in mangosteen Garcinia mangostana L.

Rahayu S., . and Kadir M. A., . and Aziz M. A., . and Rashid A. A., . (2003) The effect of different combinations of cytokinins on shoot proliferation of plant micropropagation in mangosteen Garcinia mangostana L. [Proceedings Paper]

Mangosteen Garcinia mangostana L. is an under exploited tropical fruit tree with promising economic value. Propagation of mangosteen is commonly by seeds Mangosteen fruits seasonally and produce seeds at the rate of two seeds per fruit. The development of mangosteen plant is slow and it takes about 10 - 12 years to bear fruit. The seeds are known as recalritrant. The recalritrant behavior causes difficulties in producing planting materials throughout the year. Up to now there is no suitable method for storing mangosteen seeds. Thus there exists a problem of not having enough planting materials for large scale planting. The conventional vegetative propagation method has low percentage of success. One of the alternative approaches for mass propagation of mangosteen is through tissue culture. With this technique large number of seedlings can be produced in a short time. By the method hopefully shoots can be regenerated. Thus the requirement of planting materials for mangosteen can be fulfilled. Shoot proliferation from seed segments of mangosteen has been conducted. Multiplication was achieved by culturing seed segments on Murashige and Skoog medium enriched with various concentrations of cytokinins i.e.: BAP kinetin and 100 mg/l Adenine sulfate in combination or alone 30 g/l sucrose 3.9 mg/l Gelrite agar and kept 8 weeks in culture. Shoots were obtained in all those media after 2 weeks in culture. After 8 weeks in culture there were significantly different among the treatments used on the induction of shoot. The best media for shoot proliferation was 8 mg/l BAP 11.82 shoots per explant and the highest shoot was obtained from 1 mg/l kinetin 2.27 cm.

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Abstract

Mangosteen Garcinia mangostana L. is an under exploited tropical fruit tree with promising economic value. Propagation of mangosteen is commonly by seeds Mangosteen fruits seasonally and produce seeds at the rate of two seeds per fruit. The development of mangosteen plant is slow and it takes about 10 - 12 years to bear fruit. The seeds are known as recalritrant. The recalritrant behavior causes difficulties in producing planting materials throughout the year. Up to now there is no suitable method for storing mangosteen seeds. Thus there exists a problem of not having enough planting materials for large scale planting. The conventional vegetative propagation method has low percentage of success. One of the alternative approaches for mass propagation of mangosteen is through tissue culture. With this technique large number of seedlings can be produced in a short time. By the method hopefully shoots can be regenerated. Thus the requirement of planting materials for mangosteen can be fulfilled. Shoot proliferation from seed segments of mangosteen has been conducted. Multiplication was achieved by culturing seed segments on Murashige and Skoog medium enriched with various concentrations of cytokinins i.e.: BAP kinetin and 100 mg/l Adenine sulfate in combination or alone 30 g/l sucrose 3.9 mg/l Gelrite agar and kept 8 weeks in culture. Shoots were obtained in all those media after 2 weeks in culture. After 8 weeks in culture there were significantly different among the treatments used on the induction of shoot. The best media for shoot proliferation was 8 mg/l BAP 11.82 shoots per explant and the highest shoot was obtained from 1 mg/l kinetin 2.27 cm.

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Item Type:	Proceedings Paper
Additional Information:	Received on 3 Dec 2007 QK710.2 M239 2003
AGROVOC Term:	MANGOSTEEN
AGROVOC Term:	GARCINIA MANGOSTANA
AGROVOC Term:	SHOOTS
AGROVOC Term:	CYTOKININS
AGROVOC Term:	PLANT PROPAGATION
AGROVOC Term:	SEEDS
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:28
URI:	http://webagris.upm.edu.my/id/eprint/16996

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