Extraction of alpha-amylase from Bacillus circulans SB-1 by crossflow microfiltration


Citation

Asiah O., . and Badlishah S.B., . and Asbi B.A., . Extraction of alpha-amylase from Bacillus circulans SB-1 by crossflow microfiltration. pp. 154-157. ISSN 1505-5337

Abstract

Crossflow microfiltration has been advocated as a useful tool in enzyme extraction. In this study thermostable alpha-amylase produced from a local source was microfiltered under various conditions of tangential flowrate pressure and pH. The effect of these parameters on flux and enzyme transmission was investigated. Flux was found to increase linearly with all the parameters studied and empirical equations to explain these results have been developed. Enzyme transmission was maximum at the estimated isoelectric point of the alpha-amylase.


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Abstract

Crossflow microfiltration has been advocated as a useful tool in enzyme extraction. In this study thermostable alpha-amylase produced from a local source was microfiltered under various conditions of tangential flowrate pressure and pH. The effect of these parameters on flux and enzyme transmission was investigated. Flux was found to increase linearly with all the parameters studied and empirical equations to explain these results have been developed. Enzyme transmission was maximum at the estimated isoelectric point of the alpha-amylase.

Additional Metadata

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Item Type: Article
Additional Information: Summary (En)
AGROVOC Term: BACILLUS
AGROVOC Term: ALPHA AMYLASE
AGROVOC Term: MICROFILTRATION
AGROVOC Term: CELL CULTURE
AGROVOC Term: EXTRACTS
AGROVOC Term: MALAYSIA BACILLUS
AGROVOC Term: ALFA AMILASA
AGROVOC Term: MICROFILTRACION
AGROVOC Term: CULTIVO DE CELULAS
AGROVOC Term: EXTRACTOS
Depositing User: Ms. Norfaezah Khomsan
Last Modified: 24 Apr 2025 05:52
URI: http://webagris.upm.edu.my/id/eprint/17482

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