Molecular detection, characterisation and serological survey of chicken astrovirus from broiler flocks in Malaysia

Abdullahi Raji, Abdullahi and Aini Ideris and Mohd Hair Bejo and Abdul Rahman Omar. (2022) Molecular detection, characterisation and serological survey of chicken astrovirus from broiler flocks in Malaysia. Pertanika Journal of Science & Technology (Malaysia), 30 (2). pp. 1641-1655. ISSN 2231-8526

Astroviruses have been associated with enteric and extra-intestinal disorders in many animal species, including chickens. Here, we describe the detection and characterisation of chicken astrovirus (CAstV) in broilers and its seroprevalence in broiler breeder flocks. Based on PCR protocol, viral confirmation was carried out on clinical tissue samples from broiler chickens suffering from uneven growth and poor performance. The tissues were molecularly detected for CAstV with differential diagnostic testing against the Newcastle disease virus, infectious bronchitis virus, avian nephritis virus, avian rotavirus, fowl adenovirus and avian reovirus. Polymerase gene-based phylogenetic analyses of the twenty samples detected positive for CAstV indicate they belong to Group I and are related to strains from the US, UK, India and Poland. From these 20 samples, CastV could be isolated from 3 samples upon inoculation in 5-day-old specific-pathogen-free (SPF) embryonated chicken eggs (ECE); virus-infected embryos showed dwarfing, haemorrhages, oedema and gelatinous lesions at harvest. The enzyme-linked immunosorbent assay (ELISA) results revealed a high prevalence of antibodies against CAstV amongst the broiler breeder flocks tested. It is the first study that describes the detection and prevalence of CAstV in broiler chickens and broiler breeder flocks in Malaysia.

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Abstract

Astroviruses have been associated with enteric and extra-intestinal disorders in many animal species, including chickens. Here, we describe the detection and characterisation of chicken astrovirus (CAstV) in broilers and its seroprevalence in broiler breeder flocks. Based on PCR protocol, viral confirmation was carried out on clinical tissue samples from broiler chickens suffering from uneven growth and poor performance. The tissues were molecularly detected for CAstV with differential diagnostic testing against the Newcastle disease virus, infectious bronchitis virus, avian nephritis virus, avian rotavirus, fowl adenovirus and avian reovirus. Polymerase gene-based phylogenetic analyses of the twenty samples detected positive for CAstV indicate they belong to Group I and are related to strains from the US, UK, India and Poland. From these 20 samples, CastV could be isolated from 3 samples upon inoculation in 5-day-old specific-pathogen-free (SPF) embryonated chicken eggs (ECE); virus-infected embryos showed dwarfing, haemorrhages, oedema and gelatinous lesions at harvest. The enzyme-linked immunosorbent assay (ELISA) results revealed a high prevalence of antibodies against CAstV amongst the broiler breeder flocks tested. It is the first study that describes the detection and prevalence of CAstV in broiler chickens and broiler breeder flocks in Malaysia.

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Item Type:	Article
AGROVOC Term:	broiler chickens
AGROVOC Term:	molecular characterization
AGROVOC Term:	immunodiagnosis
AGROVOC Term:	PCR
AGROVOC Term:	Avian influenza
AGROVOC Term:	poultry farming
AGROVOC Term:	sampling
AGROVOC Term:	viruses
AGROVOC Term:	bird diseases
Geographical Term:	Malaysia
Uncontrolled Keywords:	Broiler breeder, broilers, chicken astrovirus, Malaysia, PCR, polymerase gene
Depositing User:	Ms. Azariah Hashim
Date Deposited:	12 Nov 2024 04:11
Last Modified:	12 Nov 2024 04:11
URI:	http://webagris.upm.edu.my/id/eprint/1768

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