Multidrug resistant strains inhibition by Bacillus species from the gut of Oreochomis niloticus and Pomacea canaliculata


Citation

Lirio, Gary Antonio. (2022) Multidrug resistant strains inhibition by Bacillus species from the gut of Oreochomis niloticus and Pomacea canaliculata. Pertanika Journal of Science & Technology (Malaysia), 30 (2). 1657 -1688. ISSN 2231-8526

Abstract

Antibiotic resistance is widespread in clinical settings, indicating a serious problem with infectious disease treatment. Novel strategies such as using natural products derived from microbes are being explored, generating increased research interest to address this issue. Here, the antimicrobial property of gut-associated Bacillus species against multidrug-resistant (MDR) strains; methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli producing extended-spectrum beta-lactamase (EsβL E. coli), and Pseudomonas aeruginosa producing metallo beta-lactamase (MβL P.aeruginosa) was evaluated using a cross-streak method and agar diffusion assay. The Bacillus isolates inhibited MRSA and ESβL E. coli with an average zone of inhibition of 9.57 ± 33.40 mm and 5.07 ± 32.69 mm, respectively, in the cross-streak method. The cell-free supernatant (CFS) of ten Bacillus species demonstrated anti-MRSA activity but was ineffective against ESβL E. coli and MβL P. aeruginosa. The relative enzyme activities of ten Bacillus isolates were determined in vitro, and amylase, caseinase, cellulase, lipase, and gelatinase production were confirmed. Isolates were identified as Bacillus siamensis, Bacillus velezensis, and Bacillus subtilis through biochemical tests and 16s rRNA sequence analysis. Minimum inhibitory concentrations (MICs) of the CFSs against MRSA range is between 12.5 and 25%. Bacillus species isolated from fish and snail guts exhibited antibacterial activity against MRSA. Therefore, it is imperative to confirm the presence of anti-MRSA active compounds in Bacillus CFS and characterize them further to determine their suitability for antimicrobial drug development.


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Abstract

Antibiotic resistance is widespread in clinical settings, indicating a serious problem with infectious disease treatment. Novel strategies such as using natural products derived from microbes are being explored, generating increased research interest to address this issue. Here, the antimicrobial property of gut-associated Bacillus species against multidrug-resistant (MDR) strains; methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli producing extended-spectrum beta-lactamase (EsβL E. coli), and Pseudomonas aeruginosa producing metallo beta-lactamase (MβL P.aeruginosa) was evaluated using a cross-streak method and agar diffusion assay. The Bacillus isolates inhibited MRSA and ESβL E. coli with an average zone of inhibition of 9.57 ± 33.40 mm and 5.07 ± 32.69 mm, respectively, in the cross-streak method. The cell-free supernatant (CFS) of ten Bacillus species demonstrated anti-MRSA activity but was ineffective against ESβL E. coli and MβL P. aeruginosa. The relative enzyme activities of ten Bacillus isolates were determined in vitro, and amylase, caseinase, cellulase, lipase, and gelatinase production were confirmed. Isolates were identified as Bacillus siamensis, Bacillus velezensis, and Bacillus subtilis through biochemical tests and 16s rRNA sequence analysis. Minimum inhibitory concentrations (MICs) of the CFSs against MRSA range is between 12.5 and 25%. Bacillus species isolated from fish and snail guts exhibited antibacterial activity against MRSA. Therefore, it is imperative to confirm the presence of anti-MRSA active compounds in Bacillus CFS and characterize them further to determine their suitability for antimicrobial drug development.

Additional Metadata

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Item Type: Article
AGROVOC Term: Bacillus
AGROVOC Term: resistance to antibiotics
AGROVOC Term: microbial properties
AGROVOC Term: Oreochromis niloticus
AGROVOC Term: Pomacea canaliculata
AGROVOC Term: sampling
AGROVOC Term: molecular characterization
AGROVOC Term: microorganisms
AGROVOC Term: multidrug-resistant bacteria
Uncontrolled Keywords: Anti-MRSA, Bacillus species, cross-streak method, multidrug resistance, Oreochomis niloticus, Pomacea canaliculata
Depositing User: Ms. Azariah Hashim
Date Deposited: 12 Nov 2024 04:13
Last Modified: 12 Nov 2024 04:13
URI: http://webagris.upm.edu.my/id/eprint/1769

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