Cultivation of Rhodotorula toruloides using palm oil mill effluent: effect on the growth, lipid production, and waste removal

Ignatia Justine and Chin, Grace Joy Wei Lie and Yong, Wilson Thau Lym and Mailin Misson. (2022) Cultivation of Rhodotorula toruloides using palm oil mill effluent: effect on the growth, lipid production, and waste removal. Pertanika Journal of Science & Technology (Malaysia), 30 (4). 2477 -2491. ISSN 2231-8526

Using Palm Oil Mill Effluent (POME) as a medium for culturing oleaginous yeast is advantageous for simultaneous lipid production and waste removal. The organic compounds in POME can be utilised as a nutrient source for yeast growth. Rhodotorula toruloides yeast was cultivated in filtered and unfiltered raw POME as growth media in this study. The yeast growth, pH changes in media, lipid production and removal of chemical oxygen demand (COD) of Rhodotorula toruloides cultivated in POME were examined and compared to Rhodotorula toruloides grown in yeast peptone dextrose (YPD) control media. The COD level of filtered POME was reduced by nearly 50% after filtration. The biomass concentration of Rhodotorula toruloides in filtered POME surpassed the other media in the following order: filtered POME > YPD > unfiltered POME (152 mg/ml > 121 mg/ml > 37 mg/ml). The filtered POME was found favourable for yeast growth due to the minimal amount of colloidal particles and suspended solids. Meanwhile, the lipid production (4.51 %) in filtered POME was 4.8-fold higher than in control media. The water analysis indicated about 43% of COD reduction, signifying the ability of Rhodotorula toruloides to utilise nutrient components present in POME for growth. This study provides insightful knowledge on the utilisation of oleaginous yeast for simultaneous green waste disposal and sustainable microbial oil production.

Full text available from: Official URL: http://www.pertanika.upm.edu.my/resources/files/Pe...

Abstract

Using Palm Oil Mill Effluent (POME) as a medium for culturing oleaginous yeast is advantageous for simultaneous lipid production and waste removal. The organic compounds in POME can be utilised as a nutrient source for yeast growth. Rhodotorula toruloides yeast was cultivated in filtered and unfiltered raw POME as growth media in this study. The yeast growth, pH changes in media, lipid production and removal of chemical oxygen demand (COD) of Rhodotorula toruloides cultivated in POME were examined and compared to Rhodotorula toruloides grown in yeast peptone dextrose (YPD) control media. The COD level of filtered POME was reduced by nearly 50% after filtration. The biomass concentration of Rhodotorula toruloides in filtered POME surpassed the other media in the following order: filtered POME > YPD > unfiltered POME (152 mg/ml > 121 mg/ml > 37 mg/ml). The filtered POME was found favourable for yeast growth due to the minimal amount of colloidal particles and suspended solids. Meanwhile, the lipid production (4.51 %) in filtered POME was 4.8-fold higher than in control media. The water analysis indicated about 43% of COD reduction, signifying the ability of Rhodotorula toruloides to utilise nutrient components present in POME for growth. This study provides insightful knowledge on the utilisation of oleaginous yeast for simultaneous green waste disposal and sustainable microbial oil production.

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Item Type:	Article
AGROVOC Term:	Rhodotorula
AGROVOC Term:	yeasts
AGROVOC Term:	cultivation
AGROVOC Term:	effluents
AGROVOC Term:	growth rate
AGROVOC Term:	statistical methods
AGROVOC Term:	growing media
AGROVOC Term:	waste treatment
AGROVOC Term:	sustainable products
Geographical Term:	Malaysia
Uncontrolled Keywords:	Chemical oxygen demand, lipid production, palm oil mill effluent, Rhodotorula toruloides, waste treatment
Depositing User:	Ms. Azariah Hashim
Date Deposited:	12 Nov 2024 04:29
Last Modified:	12 Nov 2024 04:29
URI:	http://webagris.upm.edu.my/id/eprint/1810

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