Extraction of microsomal fraction from midguts of western spruce budworm Choristoneura occidentalis F. and rat liver and their ability to expoxidize aldrin


Citation

Rosli bin Mohamad, . Extraction of microsomal fraction from midguts of western spruce budworm Choristoneura occidentalis F. and rat liver and their ability to expoxidize aldrin. pp. 231-236. ISSN 0126-6128

Abstract

Microsomal fractions were isolated from midguts of western spruce budworm larvae and rat liver. The microsomal preparations were assayed for cytochrome P-450 content and aldrin epoxidae activity. Both microsomal preparations showed distinct spectrum of cytochrome P-450. Relative to protein content the cytochrome P-450 content of midguts of western spruce budworm was lower by 7-9 times compared to that of the rat liver. Epoxidae activity is present in the two microsomal preparations as indicated by their ability to convert aldrin to dieldrin. The activity of both microsomal preparations was inhibited by piperonyl butoxide


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Abstract

Microsomal fractions were isolated from midguts of western spruce budworm larvae and rat liver. The microsomal preparations were assayed for cytochrome P-450 content and aldrin epoxidae activity. Both microsomal preparations showed distinct spectrum of cytochrome P-450. Relative to protein content the cytochrome P-450 content of midguts of western spruce budworm was lower by 7-9 times compared to that of the rat liver. Epoxidae activity is present in the two microsomal preparations as indicated by their ability to convert aldrin to dieldrin. The activity of both microsomal preparations was inhibited by piperonyl butoxide

Additional Metadata

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Item Type: Article
Additional Information: 13 ref. Summaries (En Malay)
AGROVOC Term: CHORISTONEURA
AGROVOC Term: MAMIFEROS NOCIVOS/ ORGANULOS CITOPLASMATICOS
AGROVOC Term: ACTIVIDAD ENZIMATICA
AGROVOC Term: INSECTICIDAS
AGROVOC Term: ACCION DEL PLAGUICIDA
Depositing User: Ms. Norfaezah Khomsan
Last Modified: 24 Apr 2025 05:53
URI: http://webagris.upm.edu.my/id/eprint/18111

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