Application of response surface methodology to optimise the extraction of tea saponin from Camellia oleifera, and their verification by HPLC

Qian, C. M. and Zhang, W. T. and Luo, H. X. and Chen, J. (2022) Application of response surface methodology to optimise the extraction of tea saponin from Camellia oleifera, and their verification by HPLC. International Food Research Journal (Malaysia), 29. pp. 1339-1347. ISSN 2231 7546

Single factor combined with response surface methodology was used to optimise the process parameters of tea saponin extraction from Camellia oleifera. Four factors including material-liquid ratio, extraction temperature, extraction time, and ethanol concentration were selected as the influencing factors on the basis of single factor. The extraction rate of tea saponin was used as the response factor to analyse the response of these four factors and three levels. Results showed that extraction temperature of 81.69°C, material-liquid ratio of 1:11.85 g/mL, time of 6.17 h, and ethanol concentration of 56.69% were the best extraction conditions. The estimated yield of extraction was 7.46%. Analysis of the tea saponin samples by using high performance liquid chromatography showed that the main peak time was 6.668 min, and the absorption peaks and peaks were symmetric.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/29%20(06)%202022/10%20-...

Abstract

Single factor combined with response surface methodology was used to optimise the process parameters of tea saponin extraction from Camellia oleifera. Four factors including material-liquid ratio, extraction temperature, extraction time, and ethanol concentration were selected as the influencing factors on the basis of single factor. The extraction rate of tea saponin was used as the response factor to analyse the response of these four factors and three levels. Results showed that extraction temperature of 81.69°C, material-liquid ratio of 1:11.85 g/mL, time of 6.17 h, and ethanol concentration of 56.69% were the best extraction conditions. The estimated yield of extraction was 7.46%. Analysis of the tea saponin samples by using high performance liquid chromatography showed that the main peak time was 6.668 min, and the absorption peaks and peaks were symmetric.

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Item Type:	Article
AGROVOC Term:	saponins
AGROVOC Term:	Camellia
AGROVOC Term:	tea (plant) > tea (plant) Prefer using Camellia sinensisCamellia sinensis
AGROVOC Term:	sampling
AGROVOC Term:	quantitative analysis
AGROVOC Term:	extracts
AGROVOC Term:	high performance liquid chromatography > high performance liquid chromatography Prefer using HPLCHPLC
AGROVOC Term:	research data
Geographical Term:	China
Uncontrolled Keywords:	Camellia oleifera
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	04 Oct 2024 07:36
Last Modified:	04 Oct 2024 07:36
URI:	http://webagris.upm.edu.my/id/eprint/183

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