Citation
Adnin bin Adnan Tengku, . and Augustin M.A., . and Hasanah Mohd Ghazali, . Polyphenoloxidase from starfruit (Averrhoa carambola L.). pp. 219-224. ISSN 0126-6128
Abstract
Polyphenoloxidase (PPO) was isolated from starfruit (Averrhoa carambola L.) and purified by acetone precipitation and dialysis. The optimum pH for the acetone purified PPO catalysed oxidation of catechol was found to be pH 7.2. Heat inactivation studies showed that the enzyme was heat-labile. Half-lives for PPO inactivation at 70 degrees C and 75 degrees C were found to be 4.6 min and 3.1 min respectively The Michaelis constant (K( m)) and V( max) for catechol with PPO was 0.061 M and 20 U ml-1 respectively
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Abstract
Polyphenoloxidase (PPO) was isolated from starfruit (Averrhoa carambola L.) and purified by acetone precipitation and dialysis. The optimum pH for the acetone purified PPO catalysed oxidation of catechol was found to be pH 7.2. Heat inactivation studies showed that the enzyme was heat-labile. Half-lives for PPO inactivation at 70 degrees C and 75 degrees C were found to be 4.6 min and 3.1 min respectively The Michaelis constant (K( m)) and V( max) for catechol with PPO was 0.061 M and 20 U ml-1 respectively
Additional Metadata
| Item Type: | Article |
|---|---|
| Additional Information: | 1 table; 23 ref. Summary (En Malay) |
| AGROVOC Term: | AVERRHOA CARAMBOLA |
| AGROVOC Term: | OXIDORREDUCTASAS |
| AGROVOC Term: | AISLAMIENTO (TECNICA) |
| AGROVOC Term: | PURIFICACION/ ACETONA |
| AGROVOC Term: | PRECIPITACION ATMOSFERICA |
| AGROVOC Term: | DIALISIS |
| AGROVOC Term: | PH |
| AGROVOC Term: | CALOR |
| Depositing User: | Ms. Norfaezah Khomsan |
| Last Modified: | 24 Apr 2025 05:53 |
| URI: | http://webagris.upm.edu.my/id/eprint/18506 |
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