In vitro fermentation of mixed rumen microorganisms on molasses-based substrates

Norhani Abdullah, . In vitro fermentation of mixed rumen microorganisms on molasses-based substrates. pp. 331-337. ISSN 0126-6128

A fermentor was developed for studying the digestion pattern in vitro of rumen microorganisms of sheep fed a molasses-urea based diet. About 35 ml of strained rumen liquor withdrawn from Merino wethers adapted to a molasses-urea diet (100 g oaten chaff and molasses containing 3 urea and 0.135 mineral mix fed ad libitum) was used as the initial fermenting inoculum in the inner compartment. Molasses-urea substrate (8.333 molasses and 0.277 urea w/v) prepared in artificial saliva was infused at 3 ml h-1 into the outer compartment. Incubation was2048 carried out in a specially constructed water bath maintained at 39 degrees C and filled with CO2 gas. With these flow rates pH of fermenting fluids was maintained within physiological range with volatile fatty acids (VFA) production at 30-40 umole ml-1 h-1. Using the fermentor the effect of partial substitution of tryptone for urea as a nitrogen source on rumen microorganisms adapted to a molasses-urea based diet was studied with infusion solution consisting of 8.333 molasses 0.208 urea and 0.202 tryptone (w/v) prepared in artificial saliva. Factors related to cell yield studied were VFA and ammonia (NH) productions dry matter (DM) and CP yields. However no positive effects of protein supplementation (when compared to molasses-urea substrate) were observed in VFA productions DM and CP yields. Only NH3 levels of fermentors infused with molasses-urea substrate were significantly higher (P0.05) than protein supplemented fermen

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Abstract

A fermentor was developed for studying the digestion pattern in vitro of rumen microorganisms of sheep fed a molasses-urea based diet. About 35 ml of strained rumen liquor withdrawn from Merino wethers adapted to a molasses-urea diet (100 g oaten chaff and molasses containing 3 urea and 0.135 mineral mix fed ad libitum) was used as the initial fermenting inoculum in the inner compartment. Molasses-urea substrate (8.333 molasses and 0.277 urea w/v) prepared in artificial saliva was infused at 3 ml h-1 into the outer compartment. Incubation was2048 carried out in a specially constructed water bath maintained at 39 degrees C and filled with CO2 gas. With these flow rates pH of fermenting fluids was maintained within physiological range with volatile fatty acids (VFA) production at 30-40 umole ml-1 h-1. Using the fermentor the effect of partial substitution of tryptone for urea as a nitrogen source on rumen microorganisms adapted to a molasses-urea based diet was studied with infusion solution consisting of 8.333 molasses 0.208 urea and 0.202 tryptone (w/v) prepared in artificial saliva. Factors related to cell yield studied were VFA and ammonia (NH) productions dry matter (DM) and CP yields. However no positive effects of protein supplementation (when compared to molasses-urea substrate) were observed in VFA productions DM and CP yields. Only NH3 levels of fermentors infused with molasses-urea substrate were significantly higher (P0.05) than protein supplemented fermen

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Item Type:	Article
Additional Information:	Ill.; 1 table; 16 ref. Summaries (En Malay)
AGROVOC Term:	rsMELAZA
AGROVOC Term:	MICROORGANISMOS
AGROVOC Term:	RUMEN
AGROVOC Term:	FERMENTACION/ EQUIPO
AGROVOC Term:	OVINOS
AGROVOC Term:	JUGOS DIGESTIVOS
AGROVOC Term:	INCUBACION
AGROVOC Term:	UREA
AGROVOC Term:	NUTRI
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:54
URI:	http://webagris.upm.edu.my/id/eprint/19344

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