Analysis of carotenoids in vegetables by HPLC

Tee Siong E., . and Lim Chin Lam, . Analysis of carotenoids in vegetables by HPLC. pp. 91-99. ISSN 1505-5337

Eight vegetables were pre-treated with and without saponification and chromatographed by reversed-phase HPLC. Carotenoid peaks were identified using seven reference standards. Sample extracts were also chromatographed using a mixed magnesia and diatomaceous earth column. Fractions were eluted using stepwise increases in the proportion of acetone in hexane studied by UV-VIS absorption spectrophotometry and re-chromatographed by HPLC. The major carotenoids alpha- and beta-carotenes although there appeared to be some loss of lutein and lycopene were thus identified. Saponification removed various non-carotenoid pigments in green vegetables resulting in HPLC profiles which were easier to quantitate while prolonging the life of the HPLC column. The process did not result in a drastic loss of alpha- and beta-carotene although there appeared to be some loss of lutein which possesses no pro-vitamin A activity. Total carotenoid content of the saponified vegetables could be obtained from the absorbance reading of the extracts at 450 nm.

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Abstract

Eight vegetables were pre-treated with and without saponification and chromatographed by reversed-phase HPLC. Carotenoid peaks were identified using seven reference standards. Sample extracts were also chromatographed using a mixed magnesia and diatomaceous earth column. Fractions were eluted using stepwise increases in the proportion of acetone in hexane studied by UV-VIS absorption spectrophotometry and re-chromatographed by HPLC. The major carotenoids alpha- and beta-carotenes although there appeared to be some loss of lutein and lycopene were thus identified. Saponification removed various non-carotenoid pigments in green vegetables resulting in HPLC profiles which were easier to quantitate while prolonging the life of the HPLC column. The process did not result in a drastic loss of alpha- and beta-carotene although there appeared to be some loss of lutein which possesses no pro-vitamin A activity. Total carotenoid content of the saponified vegetables could be obtained from the absorbance reading of the extracts at 450 nm.

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Item Type:	Article
Additional Information:	13 ill.; 1 table; 16 ref.; Summary (En)
AGROVOC Term:	HORTALIZAS
AGROVOC Term:	CAROTENOIDES
AGROVOC Term:	CROMATOGRAFIA LIQUIDA ALTA PRESION
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:56
URI:	http://webagris.upm.edu.my/id/eprint/20454

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