In vitro antimicrobial activity of musk lime key lime and lemon extracts against food related pathogenic and spoilage bacteria


Citation

Saari N., . and Abu Bakar F., . and Zaman M. Z., . and Velu S., . and Mahyudin N. A., . In vitro antimicrobial activity of musk lime key lime and lemon extracts against food related pathogenic and spoilage bacteria. pp. 379-386. ISSN 22317546

Abstract

The demand for novel antimicrobial agents from natural resources has been increased worldwide for food conservation purpose. In this study antimicrobial activity of musk lime key lime and lemon were evaluated against various food borne pathogens and spoilage bacteria using disc diffusion test. Type of extraction solvent and concentration level significantly influenced the antibacterial activity of all the extracts. Ethanol extracts of musk lime key lime and lemon exhibited significant broadest inhibitory activity at 100 concentration level (pure extract) compared to water and juice extracts. 100 ethanol extracts of musk lime (39.7 mm) key lime (26.7 mm) and lemon (32.0 mm) exhibited the largest diameter of inhibition zone (DIZ) against Aeromonas veronii. 100 water extracts of musk lime (25.3 mm) key lime juice extract (23.3 mm) and water extracts of lemon (23.7 mm) was most effective against food spoilage bacteria A. veronii. The prominent results of the antimicrobial activity from lime key lime and lemon extracts may attribute them as potential natural food preservatives and could be used in pharmaceuticals field.


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Abstract

The demand for novel antimicrobial agents from natural resources has been increased worldwide for food conservation purpose. In this study antimicrobial activity of musk lime key lime and lemon were evaluated against various food borne pathogens and spoilage bacteria using disc diffusion test. Type of extraction solvent and concentration level significantly influenced the antibacterial activity of all the extracts. Ethanol extracts of musk lime key lime and lemon exhibited significant broadest inhibitory activity at 100 concentration level (pure extract) compared to water and juice extracts. 100 ethanol extracts of musk lime (39.7 mm) key lime (26.7 mm) and lemon (32.0 mm) exhibited the largest diameter of inhibition zone (DIZ) against Aeromonas veronii. 100 water extracts of musk lime (25.3 mm) key lime juice extract (23.3 mm) and water extracts of lemon (23.7 mm) was most effective against food spoilage bacteria A. veronii. The prominent results of the antimicrobial activity from lime key lime and lemon extracts may attribute them as potential natural food preservatives and could be used in pharmaceuticals field.

Additional Metadata

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Item Type: Article
AGROVOC Term: In vitro
AGROVOC Term: Antimicrobials
AGROVOC Term: Musk limes
AGROVOC Term: Lemons
AGROVOC Term: Pathogenic microorganisms
AGROVOC Term: Bacteria
AGROVOC Term: Food conservation
AGROVOC Term: Food spoilage
AGROVOC Term: Solvents
AGROVOC Term: Ethanol
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 06:26
URI: http://webagris.upm.edu.my/id/eprint/21301

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