Problems and prospects of using Sephacryl S-200 for separation of cellulase and xylanase complexes of Coprinus cinereus

Kamariah Long, . and Knapp J.S., . Problems and prospects of using Sephacryl S-200 for separation of cellulase and xylanase complexes of Coprinus cinereus. pp. 179-186. ISSN 0128-0686

The crude culture filtrates obtained from the growth of Coprinus cinereus on Solka Floc which contained cellulase and xylanase complexes were concentrated through ultrafiltration membrane (Diaflo PM 10 molecular weight cut-off of 10 000) and later fractionated through gel filtration chromatography (Sephacryl S-200). Results from gel filtration chromatography showed that the apparent molecular weight of protein from each activity peak was very much less than expected. It appeared that the gel did not act solely as a passive molecular sieve suggesting that an adsorption or electrostatic interaction might have occurred between the enzyme and the matrix of the column. Molecular weight determination from SDS-polyacrylamide gel electrophoresis (SDS-PAGE) gave a higher and more realistic molecular weight thus supporting the idea

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Abstract

The crude culture filtrates obtained from the growth of Coprinus cinereus on Solka Floc which contained cellulase and xylanase complexes were concentrated through ultrafiltration membrane (Diaflo PM 10 molecular weight cut-off of 10 000) and later fractionated through gel filtration chromatography (Sephacryl S-200). Results from gel filtration chromatography showed that the apparent molecular weight of protein from each activity peak was very much less than expected. It appeared that the gel did not act solely as a passive molecular sieve suggesting that an adsorption or electrostatic interaction might have occurred between the enzyme and the matrix of the column. Molecular weight determination from SDS-polyacrylamide gel electrophoresis (SDS-PAGE) gave a higher and more realistic molecular weight thus supporting the idea

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Item Type:	Article
Additional Information:	Summaries (En Ms)
AGROVOC Term:	COPRINUS
AGROVOC Term:	ULTRAFILTRACION
AGROVOC Term:	CROMATOGRAFIA SOBRE GEL
AGROVOC Term:	ENZIMAS
AGROVOC Term:	PESO MOLECULAR
AGROVOC Term:	CELULOSA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:27
URI:	http://webagris.upm.edu.my/id/eprint/21420

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