Analysis of ovine uterus Expressed Sequence Tags (ESTs)

Roziatul Erin A.R., . and Azlina Azma I.A., . and M. N. Mat Isa, . and Rabiatul Adawiah Z. A., . and Suhaimi A.H.M.S., . and S. Md Tasol, . and W. Somarny W. M. Z., . and K. Musaddin, . Analysis of ovine uterus Expressed Sequence Tags (ESTs). pp. 1-12. ISSN 1394-3227

The aim of this paper was to explore the different analyses available to examine Expressed Sequence Tags (ESTs). ESTs were generated from the uterus of indigenous sheep Malin. A total of 675 unigenes were identified of which 603 were singletons and 72 were consensus sequences. Seventy nine of the unigenes had significant match to genes in SwissProt database while the remaining unigenes had to be converted to coding regions (CDS). Fifty seven of the CDS had significant matches to the SwissProt database. Upon functional annotation to both unigenes and CDS binding and catalytic activity were the two most common molecular function while the most common biological processes were related to cellular processes. Enzyme commission pathway related to DNA purine and primidine metabolism pathway had the most abundant number of genes Subjected to KEGG pathway mapping. Pfam protein family related to electron transport process towards the production of ATP family was additionally identified among the ESTs. Analysis of ESTs with Pfam revealed the importance of ATP generation in the uterus. Albeit minimally the different analyses performed in this project provide some light in the function of ovine uterus and can be applied to other indigenous species that have no or limited genome data availability to discover novel and unknown genes and proteins.

Full text available from: Official URL: http://www.msap.my/mjas15/1-Husna.pdf

Abstract

The aim of this paper was to explore the different analyses available to examine Expressed Sequence Tags (ESTs). ESTs were generated from the uterus of indigenous sheep Malin. A total of 675 unigenes were identified of which 603 were singletons and 72 were consensus sequences. Seventy nine of the unigenes had significant match to genes in SwissProt database while the remaining unigenes had to be converted to coding regions (CDS). Fifty seven of the CDS had significant matches to the SwissProt database. Upon functional annotation to both unigenes and CDS binding and catalytic activity were the two most common molecular function while the most common biological processes were related to cellular processes. Enzyme commission pathway related to DNA purine and primidine metabolism pathway had the most abundant number of genes Subjected to KEGG pathway mapping. Pfam protein family related to electron transport process towards the production of ATP family was additionally identified among the ESTs. Analysis of ESTs with Pfam revealed the importance of ATP generation in the uterus. Albeit minimally the different analyses performed in this project provide some light in the function of ovine uterus and can be applied to other indigenous species that have no or limited genome data availability to discover novel and unknown genes and proteins.

[error in script]

Item Type:	Article
AGROVOC Term:	Sheep
AGROVOC Term:	Uterus
AGROVOC Term:	DNA
AGROVOC Term:	Bioinformatics
AGROVOC Term:	animal genetics
AGROVOC Term:	Genes
AGROVOC Term:	Animal protein
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:27
URI:	http://webagris.upm.edu.my/id/eprint/21552

Actions (login required)

View Item