Electrochemical sensors for detection of tetracycline antibiotics

Gayah A. R., . and Hazana R., . and Azima A., . and Nur Azura M. S., . and Faridah S., . and Zamri I., . and Norzaili Z., . Electrochemical sensors for detection of tetracycline antibiotics. pp. 67-80. ISSN 1394-3227

An electrochemical immunosensor based on screen-printed carbon working electrode with onboard carbon counter and silver“silver chloride pseudo-reference electrode for tetracycline antibiotic detection is described in this paper. A direct competitive enzyme-linked immunosorbent assay (ELISA) format was first developed and optimized on the surface of a carbon screen-printed working electrode by immobilizing the capture antibody (polyclonal) using electro-deposition of gold nanoparticles conjugated with polyclonal anti- tetracycline antibodies. The detection reagent tetracycline-Horseradish Peroxidase (HRP) conjugate was used as an enzyme label. Electrochemical detection was then carried out using 3 3 5 5-tetramethylbenzidine dihydrochloride (TMB) /H2O2 as the enzyme mediator /substrate system and conducted using chronoamperometry at 300mV vs. onboard screen-printed Ag-AgCl pseudo- reference electrode. Tetracycline detection of 10 ppb was achieved. The optimal sensor configuration was then compared to a commercial ELISA kit method for analyzing meat samples. The sensor was also capable to detect four derivatives of tetracycline tested such as Oxytetracycline Doxycline Chlortetracycline and Demeclocycline. The sensor showed good selectivity for tetracycline with higher recovery percentage (70 - 95) in the presence of other antibiotics in the sample. It also showed very high sensitivity using the amplification strategy applied with the use of gold nano-particles. This makes the developed sensor a potential tool for a rapid simple and sensitive detection of tetracycline residues in poultry products.

Full text available from: Official URL: http://www.msap.my/mjas15/8-Faridahs.pdf

Abstract

An electrochemical immunosensor based on screen-printed carbon working electrode with onboard carbon counter and silver“silver chloride pseudo-reference electrode for tetracycline antibiotic detection is described in this paper. A direct competitive enzyme-linked immunosorbent assay (ELISA) format was first developed and optimized on the surface of a carbon screen-printed working electrode by immobilizing the capture antibody (polyclonal) using electro-deposition of gold nanoparticles conjugated with polyclonal anti- tetracycline antibodies. The detection reagent tetracycline-Horseradish Peroxidase (HRP) conjugate was used as an enzyme label. Electrochemical detection was then carried out using 3 3 5 5-tetramethylbenzidine dihydrochloride (TMB) /H2O2 as the enzyme mediator /substrate system and conducted using chronoamperometry at 300mV vs. onboard screen-printed Ag-AgCl pseudo- reference electrode. Tetracycline detection of 10 ppb was achieved. The optimal sensor configuration was then compared to a commercial ELISA kit method for analyzing meat samples. The sensor was also capable to detect four derivatives of tetracycline tested such as Oxytetracycline Doxycline Chlortetracycline and Demeclocycline. The sensor showed good selectivity for tetracycline with higher recovery percentage (70 - 95) in the presence of other antibiotics in the sample. It also showed very high sensitivity using the amplification strategy applied with the use of gold nano-particles. This makes the developed sensor a potential tool for a rapid simple and sensitive detection of tetracycline residues in poultry products.

[error in script]

Item Type:	Article
AGROVOC Term:	Antibiotics
AGROVOC Term:	Antibiotic properties
AGROVOC Term:	Tetracyclines
AGROVOC Term:	ELISA
AGROVOC Term:	Competitive ELISA
AGROVOC Term:	Disease control
AGROVOC Term:	Disease treatment
AGROVOC Term:	Animal growth promoters
AGROVOC Term:	Growth
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:27
URI:	http://webagris.upm.edu.my/id/eprint/21559

Actions (login required)

View Item