Antispasmodic and nephroprotective potentials of native Algerian propolis and bee pollen: an experimental study in mice

Ali Haimoud, S. and Allem, R. and Medjekane, M. and Benyahla Djeffaland, K. and Lembarki, N. E. and Boutara, K. and Belhache, F. (2023) Antispasmodic and nephroprotective potentials of native Algerian propolis and bee pollen: an experimental study in mice. International Food Research Journal (Malaysia), 30. pp. 109-118. ISSN 2231 7546

The present work examined the in vivo antispasmodic and nephroprotective potentials of methanolic extracts obtained from Algerian native propolis and bee pollen. The in vivo antispasmodic activity was assessed by the intraperitoneal injection of acetic acid (1%) which induced long-lasting visceral pain in mice. The renal damage was modelled by intraperitoneal injection of a cisplatin (CP; 10 mg/kg) followed by histopathological changes in kidneys. In addition, the beehive by-products were screened for their bioactive content and in vitro antioxidant activities. The propolis and bee pollen are rich sources of bioactive compounds. The propolis showed the highest antioxidant potencies as evaluated by β-carotene bleaching system (87.16 ± 3.69%), DPPH (176.05 ± 0.20 µg/mL), and FRAP (0.61 ± 0.002 μmol Fe(II)/g) assays. The antispasmodic test revealed that propolis extract (250 mg/kg) significantly inhibited the number of spasms (61.04 ± 3.92%) induced by acetic acid. Based on histopathology examinations, bee pollen extract at 250 mg/kg significantly reduced nephrotoxic effects induced by CP injection. These results provided a good scientific basis for future research on antispasmodic and nephroprotective effects and/or mechanisms of propolis and bee pollen, which confer them a real application in drug discovery.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/30%20(01)%202023/08%20-...

Abstract

The present work examined the in vivo antispasmodic and nephroprotective potentials of methanolic extracts obtained from Algerian native propolis and bee pollen. The in vivo antispasmodic activity was assessed by the intraperitoneal injection of acetic acid (1%) which induced long-lasting visceral pain in mice. The renal damage was modelled by intraperitoneal injection of a cisplatin (CP; 10 mg/kg) followed by histopathological changes in kidneys. In addition, the beehive by-products were screened for their bioactive content and in vitro antioxidant activities. The propolis and bee pollen are rich sources of bioactive compounds. The propolis showed the highest antioxidant potencies as evaluated by β-carotene bleaching system (87.16 ± 3.69%), DPPH (176.05 ± 0.20 µg/mL), and FRAP (0.61 ± 0.002 μmol Fe(II)/g) assays. The antispasmodic test revealed that propolis extract (250 mg/kg) significantly inhibited the number of spasms (61.04 ± 3.92%) induced by acetic acid. Based on histopathology examinations, bee pollen extract at 250 mg/kg significantly reduced nephrotoxic effects induced by CP injection. These results provided a good scientific basis for future research on antispasmodic and nephroprotective effects and/or mechanisms of propolis and bee pollen, which confer them a real application in drug discovery.

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Item Type:	Article
AGROVOC Term:	propolis
AGROVOC Term:	pollen
AGROVOC Term:	beehives > beehives Prefer using hiveshives
AGROVOC Term:	natural antioxidants > natural antioxidants Prefer using antioxidantsantioxidants
AGROVOC Term:	laboratory animals
AGROVOC Term:	phenolic content
AGROVOC Term:	statistical methods
AGROVOC Term:	medicines > medicines Prefer using drugsdrugs
Geographical Term:	Algeria
Uncontrolled Keywords:	Antispasmodic
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	28 Oct 2024 07:13
Last Modified:	28 Oct 2024 07:13
URI:	http://webagris.upm.edu.my/id/eprint/216

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