Citation
Yusop S.M., . and Mahmoodani F., . and Babji A.S., . and Sanaei A.V., . and See S.F., . Optimization of gelatin extraction and physico-chemical properties of catfish (Clarias gariepinus) bone gelatin. pp. 423-430. ISSN 22317546
Abstract
The extraction of catfish (Clarias gariepinus) bone gelatin was optimized by using Response Surface Methodology (RSM) involving 4-factors 5-levels Central Composite Design (CCD). The optimum conditions for extraction were produced by a pre-treatment of 3.35 HCl for 14.5 h along with hot water extraction at 67.23C for 5.2 h. Results showed that the predicted yield by RSM (61.81) was closely matched the experimental yield of 60.54. The results also indicated that the extracted bone gelatin possessed high protein content (81.75) and imino acid (proline and hydroxyproline) (144 residues per 1000 residues) with gel strength (230.25 g) viscosity (4.64 mPa.s) and isoionic point (5.35) comparable to that of bovine gelatin. The results suggested that RSM is a great optimizing tool for extraction of gelatin from clarias catfish bone and values of the physicochemical properties of gelatin are higher or comparable than those from other fish species and bovine gelatin.
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Abstract
The extraction of catfish (Clarias gariepinus) bone gelatin was optimized by using Response Surface Methodology (RSM) involving 4-factors 5-levels Central Composite Design (CCD). The optimum conditions for extraction were produced by a pre-treatment of 3.35 HCl for 14.5 h along with hot water extraction at 67.23C for 5.2 h. Results showed that the predicted yield by RSM (61.81) was closely matched the experimental yield of 60.54. The results also indicated that the extracted bone gelatin possessed high protein content (81.75) and imino acid (proline and hydroxyproline) (144 residues per 1000 residues) with gel strength (230.25 g) viscosity (4.64 mPa.s) and isoionic point (5.35) comparable to that of bovine gelatin. The results suggested that RSM is a great optimizing tool for extraction of gelatin from clarias catfish bone and values of the physicochemical properties of gelatin are higher or comparable than those from other fish species and bovine gelatin.
Additional Metadata
Item Type: | Article |
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AGROVOC Term: | Gelatine |
AGROVOC Term: | Extraction |
AGROVOC Term: | Physicochemical properties |
AGROVOC Term: | Marine catfish |
AGROVOC Term: | Clarias gariepinus |
Depositing User: | Ms. Suzila Mohamad Kasim |
Last Modified: | 24 Apr 2025 06:27 |
URI: | http://webagris.upm.edu.my/id/eprint/21636 |
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