Optimization of gelatin extraction and physico-chemical properties of catfish (Clarias gariepinus) bone gelatin

Yusop S.M., . and Mahmoodani F., . and Babji A.S., . and Sanaei A.V., . and See S.F., . Optimization of gelatin extraction and physico-chemical properties of catfish (Clarias gariepinus) bone gelatin. pp. 423-430. ISSN 22317546

The extraction of catfish (Clarias gariepinus) bone gelatin was optimized by using Response Surface Methodology (RSM) involving 4-factors 5-levels Central Composite Design (CCD). The optimum conditions for extraction were produced by a pre-treatment of 3.35 HCl for 14.5 h along with hot water extraction at 67.23C for 5.2 h. Results showed that the predicted yield by RSM (61.81) was closely matched the experimental yield of 60.54. The results also indicated that the extracted bone gelatin possessed high protein content (81.75) and imino acid (proline and hydroxyproline) (144 residues per 1000 residues) with gel strength (230.25 g) viscosity (4.64 mPa.s) and isoionic point (5.35) comparable to that of bovine gelatin. The results suggested that RSM is a great optimizing tool for extraction of gelatin from clarias catfish bone and values of the physicochemical properties of gelatin are higher or comparable than those from other fish species and bovine gelatin.

Full text available from: Official URL: http://ifrj.upm.edu.my/20%20(01)%202013/59%20IFRJ%...

Abstract

The extraction of catfish (Clarias gariepinus) bone gelatin was optimized by using Response Surface Methodology (RSM) involving 4-factors 5-levels Central Composite Design (CCD). The optimum conditions for extraction were produced by a pre-treatment of 3.35 HCl for 14.5 h along with hot water extraction at 67.23C for 5.2 h. Results showed that the predicted yield by RSM (61.81) was closely matched the experimental yield of 60.54. The results also indicated that the extracted bone gelatin possessed high protein content (81.75) and imino acid (proline and hydroxyproline) (144 residues per 1000 residues) with gel strength (230.25 g) viscosity (4.64 mPa.s) and isoionic point (5.35) comparable to that of bovine gelatin. The results suggested that RSM is a great optimizing tool for extraction of gelatin from clarias catfish bone and values of the physicochemical properties of gelatin are higher or comparable than those from other fish species and bovine gelatin.

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Item Type:	Article
AGROVOC Term:	Gelatine
AGROVOC Term:	Extraction
AGROVOC Term:	Physicochemical properties
AGROVOC Term:	Marine catfish
AGROVOC Term:	Clarias gariepinus
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:27
URI:	http://webagris.upm.edu.my/id/eprint/21636

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