Effects of leptin and thyroglobulin gene polymorphisms on beef colour in Holstein bulls for slaughter in Turkey

Vapur, G. and Kök, S. (2023) Effects of leptin and thyroglobulin gene polymorphisms on beef colour in Holstein bulls for slaughter in Turkey. International Food Research Journal (Malaysia), 30. pp. 130-141. ISSN 2231 7546

In the present work, live weight (LW), hot carcass weight (HCW), and beef colour values of Turkish Holstein bull (THBs) samples, and their relationship with single nucleotide polymorphisms (SNPs) variants were determined. E2JW and E2FB variants of leptin (LEP), and C422T variant of thyroglobulin (TG) genes were determined in 100 heads of THBs by polymerase chain reaction fragment length polymorphism (PCR-RFLP). Genotyping was carried out by capillary electrophoresis. The colour of raw and cooked beefs was spectrophotometrically measured before and after cooking. The cooked beefs were significantly brighter in the LEP E2JW AA and AT variants than in the TT genotype (p < 0.05). Based on b* of raw beefs, the yellowish colour density in the LEP E2JW AA genotype variant was significantly higher than in AT and TT (p < 0.05). The most significant correlation was determined between b* and L* (0.695), and b* and a* (0.694) of raw beefs, while the correlation coefficient between LW and HCW was found to be 0.604 (p < 0.01). The LEP E2JW AA marker genotype for cattle with brighter and more intense beef, and the LEP E2JW TT variant genotype to increase beef yield should be selected as a study by using MAS method at an early age. Also, AT / CT / CC marker genotypes of THBs should be selected in LEP E2JW / E2FB / TG C422T marker loci, respectively to generate more income from the increase in LW and HCW.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/30%20(01)%202023/10%20-...

Abstract

In the present work, live weight (LW), hot carcass weight (HCW), and beef colour values of Turkish Holstein bull (THBs) samples, and their relationship with single nucleotide polymorphisms (SNPs) variants were determined. E2JW and E2FB variants of leptin (LEP), and C422T variant of thyroglobulin (TG) genes were determined in 100 heads of THBs by polymerase chain reaction fragment length polymorphism (PCR-RFLP). Genotyping was carried out by capillary electrophoresis. The colour of raw and cooked beefs was spectrophotometrically measured before and after cooking. The cooked beefs were significantly brighter in the LEP E2JW AA and AT variants than in the TT genotype (p < 0.05). Based on b* of raw beefs, the yellowish colour density in the LEP E2JW AA genotype variant was significantly higher than in AT and TT (p < 0.05). The most significant correlation was determined between b* and L* (0.695), and b* and a* (0.694) of raw beefs, while the correlation coefficient between LW and HCW was found to be 0.604 (p < 0.01). The LEP E2JW AA marker genotype for cattle with brighter and more intense beef, and the LEP E2JW TT variant genotype to increase beef yield should be selected as a study by using MAS method at an early age. Also, AT / CT / CC marker genotypes of THBs should be selected in LEP E2JW / E2FB / TG C422T marker loci, respectively to generate more income from the increase in LW and HCW.

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Item Type:	Article
AGROVOC Term:	bulls
AGROVOC Term:	beef
AGROVOC Term:	sampling
AGROVOC Term:	coloration > coloration Prefer using colourcolour
AGROVOC Term:	statistical methods
AGROVOC Term:	carcass weight
AGROVOC Term:	SNP > SNP Prefer using single nucleotide polymorphismsingle nucleotide polymorphism
AGROVOC Term:	meat quality
Geographical Term:	Turkey
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	29 Oct 2024 01:48
Last Modified:	29 Oct 2024 01:48
URI:	http://webagris.upm.edu.my/id/eprint/218

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