Citation
Shahdan I. A., . and Rahman M. T., . Effect of pre-slaughter stunning on the death of the poultry and myofiber apoptosis. pp. 2279-2284. ISSN 22317546
Abstract
The effectiveness of poultry stunning in producing swift slaughtering was analysed in response to the time needed for the chickens to become insensible upon neck cutting (Td) and the induction of myofiber apoptosis. In total 49 chicken broilers (BW of 2.17 .24 kg) were sacrificed with pre-slaughter stunning using a constant voltage stunner where the electric current varied between 7.2 to 124.3 mA and without stunning. The electric current applied during stunning was found to have no effect on Td. Number of apoptotic myonuclei did not vary among stunned and unstunned meat. Apoptosis inducing factor (AIF) and caspase 3 expressions were also not detected in the meat samples of both stunned and unstunned groups at 1 d postmortem. Since the slaughtering process and stunning are associated with stress the expression of 70 kDa-heat shock protein (Hsp70) was investigated. Moreover Hsp70 is also an inhibitor of apoptosis by preventing the activation of AIF and apoptosome which stimulates caspase 3 activation. However expression of Hsp70 was not induced in both stunned groups and unstunned groups. Together this study found that poultry stunning does not affect Td and myofiber apoptosis.
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Abstract
The effectiveness of poultry stunning in producing swift slaughtering was analysed in response to the time needed for the chickens to become insensible upon neck cutting (Td) and the induction of myofiber apoptosis. In total 49 chicken broilers (BW of 2.17 .24 kg) were sacrificed with pre-slaughter stunning using a constant voltage stunner where the electric current varied between 7.2 to 124.3 mA and without stunning. The electric current applied during stunning was found to have no effect on Td. Number of apoptotic myonuclei did not vary among stunned and unstunned meat. Apoptosis inducing factor (AIF) and caspase 3 expressions were also not detected in the meat samples of both stunned and unstunned groups at 1 d postmortem. Since the slaughtering process and stunning are associated with stress the expression of 70 kDa-heat shock protein (Hsp70) was investigated. Moreover Hsp70 is also an inhibitor of apoptosis by preventing the activation of AIF and apoptosome which stimulates caspase 3 activation. However expression of Hsp70 was not induced in both stunned groups and unstunned groups. Together this study found that poultry stunning does not affect Td and myofiber apoptosis.
Additional Metadata
Item Type: | Article |
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AGROVOC Term: | Poultry |
AGROVOC Term: | Slaughtering |
AGROVOC Term: | Stunning |
AGROVOC Term: | Apoptosis |
Geographical Term: | Malaysia |
Depositing User: | Ms. Suzila Mohamad Kasim |
Last Modified: | 26 Apr 2025 15:55 |
URI: | http://webagris.upm.edu.my/id/eprint/21932 |
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