Occurrence of Shiga toxin-producing Escherichia coli (STEC) in bovine feces feed water raw milk pasteurized milk Minas Frescal cheese and ground beef samples collected in Minas Gerais Brazil

Silva F. J. M., . and Oliveira P. M., . and Martins M. L., . and Santos M. T., . and Leite Júnior B. R. C., . and Moraes C. A., . and Gomes A. P., . and Júnior A. S., . Occurrence of Shiga toxin-producing Escherichia coli (STEC) in bovine feces feed water raw milk pasteurized milk Minas Frescal cheese and ground beef samples collected in Minas Gerais Brazil. pp. 2481-2486. ISSN 22317546

This study aimed to evaluate the occurrence of Shiga toxin-producing Escherichia coli (STEC) in feces samples water feed raw milk pasteurized milk Minas Frescal cheese and ground beef. Seventy samples (40 of feces 10 of raw milk 5 of pasteurized milk 5 of ground beef 4 of Minas Frescal cheese 4 of water and 2 of feed) were collected in two farms of dairy cattle and two farms of beef cattle and in commercial establishments. The samples were processed according to standard methodologies using biochemical methods and molecular and biological assays. Among 209 identified bacterial isolates as E. coli 120 70 7 7 3 2 and zero were obtained from samples of feces water feed ground beef pasteurized milk Minas Frescal cheese and raw milk respectively. An isolate from water sample coming from a beef cattle farm was identified as E. coli STEC. This isolate showed cytotoxic effect in Vero cells and the EhlyA gene encoding entero-hemolysin. Therefore it is justified to develop studies to limit the spread of this pathogen.

Full text available from: Official URL: http://ifrj.upm.edu.my/21%20(06)%202014/58%20IFRJ%...

Abstract

This study aimed to evaluate the occurrence of Shiga toxin-producing Escherichia coli (STEC) in feces samples water feed raw milk pasteurized milk Minas Frescal cheese and ground beef. Seventy samples (40 of feces 10 of raw milk 5 of pasteurized milk 5 of ground beef 4 of Minas Frescal cheese 4 of water and 2 of feed) were collected in two farms of dairy cattle and two farms of beef cattle and in commercial establishments. The samples were processed according to standard methodologies using biochemical methods and molecular and biological assays. Among 209 identified bacterial isolates as E. coli 120 70 7 7 3 2 and zero were obtained from samples of feces water feed ground beef pasteurized milk Minas Frescal cheese and raw milk respectively. An isolate from water sample coming from a beef cattle farm was identified as E. coli STEC. This isolate showed cytotoxic effect in Vero cells and the EhlyA gene encoding entero-hemolysin. Therefore it is justified to develop studies to limit the spread of this pathogen.

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Item Type:	Article
AGROVOC Term:	Escherichia coli
AGROVOC Term:	Feces
AGROVOC Term:	Water
AGROVOC Term:	Feeds
AGROVOC Term:	Raw milk
AGROVOC Term:	Pasteurized milk
AGROVOC Term:	Cheese
AGROVOC Term:	Beef cattle
AGROVOC Term:	biochemical compounds
AGROVOC Term:	Biological markers (molecular)
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:27
URI:	http://webagris.upm.edu.my/id/eprint/21959

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