Antioxidant activity and functional properties of fractionated cobia skin gelatin hydrolysate at different molecular weight


Citation

Razali A. N., . and Amin A. M., . and Sarbon N. M., . Antioxidant activity and functional properties of fractionated cobia skin gelatin hydrolysate at different molecular weight. pp. 651-660. ISSN 22317546

Abstract

This study investigated the antioxidant activity and functional properties of fractionated cobia skin gelatin hydrolysate (CSGH) at different molecular weights (10 5 and 3 kDa). Antioxidant activities studied included reducing power ferrous ion chelation DPPH (1 1- diphenyl-2-picrylhydrazyl) radical scavenging and superoxide anion scavenging. Functional properties studied included emulsifying and foaming properties as well as fat and water binding capacity. Results showed significant differences (p 0.05) between the chelating activity of ferrous and superoxide anions for 5 kDa and other fractions while the highest DPPH radical scavenging activity was presented by the 3 kDa fraction. The 10 kDa fraction had the highest fat binding capacity (28 2) while functional properties were not affected by lower molecular weight. Lower outcomes obtained for functional properties from smaller molecular weights may have been due to enzymatic hydrolysis and the fractionation process to which the hydrolysate was subjected. Hence varied CSGH molecular weights appeared to benefit different functions.


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Abstract

This study investigated the antioxidant activity and functional properties of fractionated cobia skin gelatin hydrolysate (CSGH) at different molecular weights (10 5 and 3 kDa). Antioxidant activities studied included reducing power ferrous ion chelation DPPH (1 1- diphenyl-2-picrylhydrazyl) radical scavenging and superoxide anion scavenging. Functional properties studied included emulsifying and foaming properties as well as fat and water binding capacity. Results showed significant differences (p 0.05) between the chelating activity of ferrous and superoxide anions for 5 kDa and other fractions while the highest DPPH radical scavenging activity was presented by the 3 kDa fraction. The 10 kDa fraction had the highest fat binding capacity (28 2) while functional properties were not affected by lower molecular weight. Lower outcomes obtained for functional properties from smaller molecular weights may have been due to enzymatic hydrolysis and the fractionation process to which the hydrolysate was subjected. Hence varied CSGH molecular weights appeared to benefit different functions.

Additional Metadata

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Item Type: Article
AGROVOC Term: Rachycentron canadum
AGROVOC Term: Fish
AGROVOC Term: Gelatin
AGROVOC Term: Protein hydrolysate
AGROVOC Term: Molecular weight
AGROVOC Term: Antioxidants
AGROVOC Term: Enzymatic hydrolysis
AGROVOC Term: Fractionation
AGROVOC Term: Emulsifying
AGROVOC Term: Foaming
Geographical Term: Malaysia
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 26 Apr 2025 16:38
URI: http://webagris.upm.edu.my/id/eprint/22073

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