Citation
Roslan J., . and Mustapa Kamal S. M., . and Md. Yunos K. F., . and Abdullah N., . Optimization of enzymatic hydrolysis of tilapia (Oreochromis niloticus) by-product using response surface methodology. pp. 1117-1123. ISSN 22317546
Abstract
Fish protein hydrolysate was recovered from tilapia by-product (TB) through enzymatic hydrolysis using alcalase enzyme. Hydrolysis reaction of TB was monitored according to the degree of hydrolysis (DH) by employing O-phtaldialdehyde (OPA) method. Optimization process for obtaining high yield of TB protein hydrolysate was performed using response surface methodology (RSM) by optimizing a combination of four independent variables namely pH (6.5-8.5) temperature (55-70oC) substrate concentration (10-17.5 w/v) and enzyme concentration (1.5-3.5 w/w) with (DH) as a response. The optimum enzymatic hydrolysis conditions were obtained at pH 7.5 temperature of 60oC substrate concentration of 15 (w/v) and 2.5 (w/w) of enzyme concentration and yielded about 20.20 of DH after hydrolyzing for 120 min. RSM generated model predicted that 20.42 of DH could be achieved at these conditions and this model was valid based on the DH value obtained from the experimental study (20.31) which was quite similar with the predicted value. High yield of DH obtained from the optimization process could produce fish protein hydrolysate with good nutritional and functional properties.
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Abstract
Fish protein hydrolysate was recovered from tilapia by-product (TB) through enzymatic hydrolysis using alcalase enzyme. Hydrolysis reaction of TB was monitored according to the degree of hydrolysis (DH) by employing O-phtaldialdehyde (OPA) method. Optimization process for obtaining high yield of TB protein hydrolysate was performed using response surface methodology (RSM) by optimizing a combination of four independent variables namely pH (6.5-8.5) temperature (55-70oC) substrate concentration (10-17.5 w/v) and enzyme concentration (1.5-3.5 w/w) with (DH) as a response. The optimum enzymatic hydrolysis conditions were obtained at pH 7.5 temperature of 60oC substrate concentration of 15 (w/v) and 2.5 (w/w) of enzyme concentration and yielded about 20.20 of DH after hydrolyzing for 120 min. RSM generated model predicted that 20.42 of DH could be achieved at these conditions and this model was valid based on the DH value obtained from the experimental study (20.31) which was quite similar with the predicted value. High yield of DH obtained from the optimization process could produce fish protein hydrolysate with good nutritional and functional properties.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Oreochromis niloticus |
| AGROVOC Term: | Hydrolysis |
| AGROVOC Term: | Optimization methods |
| AGROVOC Term: | Temperature |
| AGROVOC Term: | Enzymes |
| AGROVOC Term: | Peptides |
| AGROVOC Term: | Pharmaceutical products |
| AGROVOC Term: | Enzyme activity |
| AGROVOC Term: | Spectrophotometers |
| AGROVOC Term: | Tilapia |
| Geographical Term: | Malaysia |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 26 Apr 2025 16:38 |
| URI: | http://webagris.upm.edu.my/id/eprint/22093 |
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