Characterization and application of alkaline -amylase from Bacillus licheniformis MTCC1483 as a detergent additive

Rathi B., . and Dahiya P., . Characterization and application of alkaline -amylase from Bacillus licheniformis MTCC1483 as a detergent additive. pp. 1293-1297. ISSN 22317546

Alpha-amylases (1-4 D- glucano hydrolase EC 3.2.1.1) are endoenzymes that hydrolyses “1 4- glycosidic linkage in starch and related substrate in an endo-fashion producing oligosaccharides glucose and “limit dextrin. To cater the constant growing need of this enzyme in detergent industrial purposes alkaline amylase secreted by Bacillus licheniformis MTCC1483 was studied in order to test its suitability as a detergent additive. It was found to produce 21.6 U/ml of alkaline amylase after 24 hours of incubation. The amylase was further characterized with respect to pH and temperature optima and stability detergent compatibility. The optimum pH and temperature for amylase activity was found to be 8.0 and 40C. The enzyme was found to retain around 80 residual activity in the pH range of pH 6.0-10.0. The enzyme showed highest stability at 40C for 1 h of incubation while at 60C 70 of the original activity was lost respectively. Compatibility of enzyme amylase with certain commercial laundry detergents was also shown to be good as enzyme retained up to 100 85 and 55 of their activities after 30 min of incubation at 40C. Supplementation of the enzyme to the detergent Tide improved the cleansing ability of the detergent with maximum strain removal in terry cloth followed by cotton fabric stained with potato curry sample. All these properties make this amylase an ideal choice for application in detergent formulations.

Full text available from: Official URL: http://ifrj.upm.edu.my/22%20(03)%202015/(57).pdf

Abstract

Alpha-amylases (1-4 D- glucano hydrolase EC 3.2.1.1) are endoenzymes that hydrolyses “1 4- glycosidic linkage in starch and related substrate in an endo-fashion producing oligosaccharides glucose and “limit dextrin. To cater the constant growing need of this enzyme in detergent industrial purposes alkaline amylase secreted by Bacillus licheniformis MTCC1483 was studied in order to test its suitability as a detergent additive. It was found to produce 21.6 U/ml of alkaline amylase after 24 hours of incubation. The amylase was further characterized with respect to pH and temperature optima and stability detergent compatibility. The optimum pH and temperature for amylase activity was found to be 8.0 and 40C. The enzyme was found to retain around 80 residual activity in the pH range of pH 6.0-10.0. The enzyme showed highest stability at 40C for 1 h of incubation while at 60C 70 of the original activity was lost respectively. Compatibility of enzyme amylase with certain commercial laundry detergents was also shown to be good as enzyme retained up to 100 85 and 55 of their activities after 30 min of incubation at 40C. Supplementation of the enzyme to the detergent Tide improved the cleansing ability of the detergent with maximum strain removal in terry cloth followed by cotton fabric stained with potato curry sample. All these properties make this amylase an ideal choice for application in detergent formulations.

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Item Type:	Article
AGROVOC Term:	Alpha amylase
AGROVOC Term:	Bacillus licheniformis
AGROVOC Term:	Enzymatic hydrolysis
AGROVOC Term:	Oligosaccharides
AGROVOC Term:	Glucose
AGROVOC Term:	Dextrins
AGROVOC Term:	Detergents
AGROVOC Term:	Incubation of microorganisms
AGROVOC Term:	Enzymatic activity
AGROVOC Term:	Enzyme activators
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:27
URI:	http://webagris.upm.edu.my/id/eprint/22116

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