Citation
Phua A. C. Y., . and Rahman M. M., . and Wan Khadijah W. E., . and Abdullah R. B., . Influence of in vitro maturation duration on embryo yield in goats. pp. 23-28. ISSN 1394-3277
Abstract
Oocytes retrieved from goat ovaries are usually still in immature stage and thus are not ready for fertlisation. Hence the oocytes need to be cultured in maturation medium for certain duration before they fertilise. In this experiment the effect of in vitro maturation duration on laparoscopic oocyte pick-up (LOPU)-derived caprine oocytes towards embryo yield (4-cell stage taken as the minimum criterion) was investigated. Separate batches of oocytes were matured for 18 to 20 h 21 to 23 h 24 to 26 h and 27 to 29 h respectively at 38.5C under 5 CO2 in humidified air before they were in vitro fertilised and cultured. LOPU-derived oocytes matured for 18 to 20 h improved (p0.01) embryo yield (42.6). In conclusion appropriate IVM duration will improve the in vitro fertilsation performance in goats.
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Abstract
Oocytes retrieved from goat ovaries are usually still in immature stage and thus are not ready for fertlisation. Hence the oocytes need to be cultured in maturation medium for certain duration before they fertilise. In this experiment the effect of in vitro maturation duration on laparoscopic oocyte pick-up (LOPU)-derived caprine oocytes towards embryo yield (4-cell stage taken as the minimum criterion) was investigated. Separate batches of oocytes were matured for 18 to 20 h 21 to 23 h 24 to 26 h and 27 to 29 h respectively at 38.5C under 5 CO2 in humidified air before they were in vitro fertilised and cultured. LOPU-derived oocytes matured for 18 to 20 h improved (p0.01) embryo yield (42.6). In conclusion appropriate IVM duration will improve the in vitro fertilsation performance in goats.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Goats |
| AGROVOC Term: | Animal embryos |
| AGROVOC Term: | Maturation |
| AGROVOC Term: | In vitro |
| AGROVOC Term: | Oocytes |
| AGROVOC Term: | Developmental stages |
| AGROVOC Term: | Oestrus |
| AGROVOC Term: | Drugs |
| AGROVOC Term: | Vagina |
| AGROVOC Term: | Superovulated females |
| Geographical Term: | Malaysia |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 26 Apr 2025 16:45 |
| URI: | http://webagris.upm.edu.my/id/eprint/22160 |
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