Citation
Watanabe A., . and Widyatmoko A., . and Rimbawanto A., . and Shiraishi S., . Discrimination of teak (Tectona grandis) plus trees using selected random amplified polymorphic DNA (RAPD) markers. pp. 17-24. ISSN 0128-1283
Abstract
To achieve a highly reliable clone management of leak (Tectona grandis) plus trees useful DNA molecular markers were surveyed using RAPD analysis and their ability to discriminate among plus tree clones was examined. A primary screening was performed for 120 arbitrary decamer primers and 24 primers that generated 26 clear and unambiguous fragments were selected. In the secondary screening the reproducibility of each fragment was investigated by six repetitions of polymerase chain reaction and 13 fragments found to be most reproducible were finally selected. Evaluation of the discriminatory powers of these fragments suggested that the selected RAPD markers would be useful in the clone management of teak plus trees.
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Abstract
To achieve a highly reliable clone management of leak (Tectona grandis) plus trees useful DNA molecular markers were surveyed using RAPD analysis and their ability to discriminate among plus tree clones was examined. A primary screening was performed for 120 arbitrary decamer primers and 24 primers that generated 26 clear and unambiguous fragments were selected. In the secondary screening the reproducibility of each fragment was investigated by six repetitions of polymerase chain reaction and 13 fragments found to be most reproducible were finally selected. Evaluation of the discriminatory powers of these fragments suggested that the selected RAPD markers would be useful in the clone management of teak plus trees.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Teak |
| AGROVOC Term: | Tectona grandis |
| AGROVOC Term: | RAPD |
| AGROVOC Term: | Random amplified polymorphic DNA |
| AGROVOC Term: | Clones |
| AGROVOC Term: | Molecular markers |
| AGROVOC Term: | Verbenaceae |
| AGROVOC Term: | PCR |
| AGROVOC Term: | Denaturation |
| AGROVOC Term: | Genetic diversity within species |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:27 |
| URI: | http://webagris.upm.edu.my/id/eprint/22177 |
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