Identification of predominant Lactobacillus species in liquid sourdough fermentation


Citation

Kam W.Y., . and Wan Aida W.M., . and Sahilah A.M., . Identification of predominant Lactobacillus species in liquid sourdough fermentation. pp. 1739-1743. ISSN 2231-7546

Abstract

Two high protein wheat flour samples of Red Horse (RH) and Bake with Yen (BY) were examined for predominant Lactobacillus spp. in fermented liquid sourdough. The identification of Lactobacillus spp. was based on biochemical tests of catalase test gas carbon dioxide production arginine test the ability to grow at temperature of 15C and 45C and carbohydrate fermentation using API50CH kit. Those strains were identified as Lactobacillus spp. and confirmed using polymerase chain reaction (PCR) of 16S rRNA partial sequencing analysis. In the present study we successfully isolated and identified the Lactobacillus plantarum and L. fermentum which were predominant bacteria in liquid sourdough of the sample RH and BY brand respectively.


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Abstract

Two high protein wheat flour samples of Red Horse (RH) and Bake with Yen (BY) were examined for predominant Lactobacillus spp. in fermented liquid sourdough. The identification of Lactobacillus spp. was based on biochemical tests of catalase test gas carbon dioxide production arginine test the ability to grow at temperature of 15C and 45C and carbohydrate fermentation using API50CH kit. Those strains were identified as Lactobacillus spp. and confirmed using polymerase chain reaction (PCR) of 16S rRNA partial sequencing analysis. In the present study we successfully isolated and identified the Lactobacillus plantarum and L. fermentum which were predominant bacteria in liquid sourdough of the sample RH and BY brand respectively.

Additional Metadata

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Item Type: Article
AGROVOC Term: Wheat flour
AGROVOC Term: Lactobacillus
AGROVOC Term: Sour doughs
AGROVOC Term: Carbon dioxide
AGROVOC Term: Lactic acid bacteria
AGROVOC Term: Lactic fermentation
AGROVOC Term: Proteolysis
AGROVOC Term: Antibacterial agents
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 06:27
URI: http://webagris.upm.edu.my/id/eprint/22274

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