Characterization of Edwardsiella tarda isolated from Asian Seabass Lates calcarifer

Nadirah M., . and Najiah M., . and Teng S. Y., . Characterization of Edwardsiella tarda isolated from Asian Seabass Lates calcarifer. pp. 1247-1252. ISSN 2231-7546

This study described the antibiotic and heavy metal resistance pattern of 17 isolates of Edwardsiella tarda obtained from Asian seabass (Lates calcarifer). E.tarda isolates were resistant to oleandomycin lincomycin novobiocin and spiramycin. In contrast most of the isolates showed high level of susceptibility to tetracycline doxycycline florfenicol chloramplenicol nitrofurantoin fosfomycin kanamycin oxolinic acid and flumequine. MAR value was 0.35 which indicated that the cultured Asian seabass have received high exposure to those tested antibiotics. Besides very high level of heavy metal resistance among these isolates was observed. Genotypic profile of DNA fingerprintings generated by RAPD-PCR using M13 universal primer and M13 wild type phage primer showed high degree of genetic diversity with percentages similarity and genetic distance among the isolates were ranging from 10.5 to 100 and 0 to 0.895 respectively. This result indicates that strains that belong to the same origin were not always closely related genetically.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/19%20(03)%202012/(63)%2...

Abstract

This study described the antibiotic and heavy metal resistance pattern of 17 isolates of Edwardsiella tarda obtained from Asian seabass (Lates calcarifer). E.tarda isolates were resistant to oleandomycin lincomycin novobiocin and spiramycin. In contrast most of the isolates showed high level of susceptibility to tetracycline doxycycline florfenicol chloramplenicol nitrofurantoin fosfomycin kanamycin oxolinic acid and flumequine. MAR value was 0.35 which indicated that the cultured Asian seabass have received high exposure to those tested antibiotics. Besides very high level of heavy metal resistance among these isolates was observed. Genotypic profile of DNA fingerprintings generated by RAPD-PCR using M13 universal primer and M13 wild type phage primer showed high degree of genetic diversity with percentages similarity and genetic distance among the isolates were ranging from 10.5 to 100 and 0 to 0.895 respectively. This result indicates that strains that belong to the same origin were not always closely related genetically.

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Item Type:	Article
AGROVOC Term:	Edwardsiella
AGROVOC Term:	Lates calcarifer
AGROVOC Term:	Antibiotics
AGROVOC Term:	Heavy metals
AGROVOC Term:	Tetracyclines
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:27
URI:	http://webagris.upm.edu.my/id/eprint/22370

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