Effect of germination on antioxidant activity total phenolics -carotene ascorbic acid and -tocopherol contents of lead tree sprouts (Leucaena leucocephala (lmk.) de Wit)

Suryanti V., . and Marliyana S. D., . and Putri H. E., . Effect of germination on antioxidant activity total phenolics -carotene ascorbic acid and -tocopherol contents of lead tree sprouts (Leucaena leucocephala (lmk.) de Wit). pp. 167-172. ISSN 2231-7546

This study aims to determine the effect of germination time on the antioxidant activity of Leucaena leucocephala (lmk.) de Wit (lead tree) sprouts. Determination of antioxidant activity was carried out on ethanol extract of germinated seeds using -carotene bleaching assay. The results showed that the antioxidant activity of germinated seeds were affected by germination time. Germination for 4 days affected the greatest enhancement in antioxidant activity by 2.78 fold and caused a significant increment in total phenolic content by 7.29 -carotene by 3.27 ascorbic acid by 3.81 and -tocopherol by 4.58 fold respect to imbibed seeds. The total phenolics content was 53.420.22 mg CAE/g dw -carotene was 530.9971.13 10-3 mg/100 g ascorbic acid was 152.372.06 mg/100 g and -tocopherol was 59.270.10 mg/100 g sample. The findings suggested that the L. leucocephala sprouts could be considered as a source of natural antioxidant.

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Abstract

This study aims to determine the effect of germination time on the antioxidant activity of Leucaena leucocephala (lmk.) de Wit (lead tree) sprouts. Determination of antioxidant activity was carried out on ethanol extract of germinated seeds using -carotene bleaching assay. The results showed that the antioxidant activity of germinated seeds were affected by germination time. Germination for 4 days affected the greatest enhancement in antioxidant activity by 2.78 fold and caused a significant increment in total phenolic content by 7.29 -carotene by 3.27 ascorbic acid by 3.81 and -tocopherol by 4.58 fold respect to imbibed seeds. The total phenolics content was 53.420.22 mg CAE/g dw -carotene was 530.9971.13 10-3 mg/100 g ascorbic acid was 152.372.06 mg/100 g and -tocopherol was 59.270.10 mg/100 g sample. The findings suggested that the L. leucocephala sprouts could be considered as a source of natural antioxidant.

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Item Type:	Article
AGROVOC Term:	Leucaena leucocephala
AGROVOC Term:	Germination
AGROVOC Term:	Phenolic acids
AGROVOC Term:	Carotenes
AGROVOC Term:	Ascorbic acid
AGROVOC Term:	Tocopherols
AGROVOC Term:	Natural antioxidants
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:27
URI:	http://webagris.upm.edu.my/id/eprint/22467

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