The use of antibody-sensitized latex to detect Cymbidium mosaic virus in orchids


Citation

Norani Abdul-Samad, . and Zainab Ari, . The use of antibody-sensitized latex to detect Cymbidium mosaic virus in orchids. pp. 157-160. ISSN 0126-6128

Abstract

Latex agglutination test (L) and a modification of the latex test protein A-coated latex (PAL) were compared for sensitization with antibodies to Cymbidium mosaic virus (CyMV). A series of globulin concentrations was tested against purified CyMV. The minimum detectable virus concentration was similar for both L and PAL reactions at 0.104 \Xg/ml The detectable end points in infected Oncidium Gower Ramsey orchid sap were similar at a dilution of 1/2560. PAL was found to be more sensitive than L at a higher globulin dilution of 1/512. The PAL test showed higher sensitivity than L based on five orchid hybrids and compared favourably with electron microscopy test.


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Abstract

Latex agglutination test (L) and a modification of the latex test protein A-coated latex (PAL) were compared for sensitization with antibodies to Cymbidium mosaic virus (CyMV). A series of globulin concentrations was tested against purified CyMV. The minimum detectable virus concentration was similar for both L and PAL reactions at 0.104 \Xg/ml The detectable end points in infected Oncidium Gower Ramsey orchid sap were similar at a dilution of 1/2560. PAL was found to be more sensitive than L at a higher globulin dilution of 1/512. The PAL test showed higher sensitivity than L based on five orchid hybrids and compared favourably with electron microscopy test.

Additional Metadata

[error in script]
Item Type: Article
AGROVOC Term: Orchidaceae
AGROVOC Term: cymbidium
AGROVOC Term: Plant viruses
AGROVOC Term: Latex agglutination test
AGROVOC Term: Globulins
AGROVOC Term: Latex
AGROVOC Term: Antiserum
Geographical Term: Malaysia
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 27 Apr 2025 23:31
URI: http://webagris.upm.edu.my/id/eprint/22767

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