Cross Species Amplification of Ikan Kelah Tor tambroides by Using Mystus nemurus Microsatellite Markers

Bun Poh Keong, . and Siti Shapor Siraj, . and S.K. Daud, . Cross Species Amplification of Ikan Kelah Tor tambroides by Using Mystus nemurus Microsatellite Markers. pp. 179-189. ISSN 1511-3701

Thirty eight microsatellite markers developed from a Random Hybridising Microsatellite (RAMs) enrichment protocol created from the DNA of the river catfish ikan baung Mystus nemurus were screened to cross amplify ikan kelah Tor tambroides. Only five primers which amplified bands at the expected allele size regions were used for characterizing this species. The observed heterozygosity values were higher than the expected heterozygosity values for the bands amplified by primer pairs MnSC4-3B MnLR2-1-52A and MnRmC3-1 across the three populations but the bands amplified by primer pairs MnSC4-1A and MnLR2-1-17B showed lower observed heterozygosity values than the expected heterozygosity values. The mean FIS value across the three populations was negative indicating no deficit in heterozygosity. The mean value of FST was low indicating no gene was fixed within populations relative to the total population. The high value of Nm suggested high gene flow among the three populations. Both the (x2) chi-square and the (G2) likelihood ratio tests showed significant differences (P0.05) indicating deviations from Hardy-Weinberg equilibrium in most loci except for one locus (MnSC4-1A) in the Negeri Sembilan population and two loci (MnLR2-1-52A and MnSC4-1A) in the Kelantan population. The genetic distance values generated ranged from 0.1053 to 0.1960. The UPGMA dendrograms constructed from the genetic distances based on the microsatellite markers showed that the Negeri Sembilan and Kelantan populations shared a similar cluster while the Pahang population was on its own.

Full text available from: Official URL: http://www.pertanika.upm.edu.my/resources/files/Pe...

Abstract

Thirty eight microsatellite markers developed from a Random Hybridising Microsatellite (RAMs) enrichment protocol created from the DNA of the river catfish ikan baung Mystus nemurus were screened to cross amplify ikan kelah Tor tambroides. Only five primers which amplified bands at the expected allele size regions were used for characterizing this species. The observed heterozygosity values were higher than the expected heterozygosity values for the bands amplified by primer pairs MnSC4-3B MnLR2-1-52A and MnRmC3-1 across the three populations but the bands amplified by primer pairs MnSC4-1A and MnLR2-1-17B showed lower observed heterozygosity values than the expected heterozygosity values. The mean FIS value across the three populations was negative indicating no deficit in heterozygosity. The mean value of FST was low indicating no gene was fixed within populations relative to the total population. The high value of Nm suggested high gene flow among the three populations. Both the (x2) chi-square and the (G2) likelihood ratio tests showed significant differences (P0.05) indicating deviations from Hardy-Weinberg equilibrium in most loci except for one locus (MnSC4-1A) in the Negeri Sembilan population and two loci (MnLR2-1-52A and MnSC4-1A) in the Kelantan population. The genetic distance values generated ranged from 0.1053 to 0.1960. The UPGMA dendrograms constructed from the genetic distances based on the microsatellite markers showed that the Negeri Sembilan and Kelantan populations shared a similar cluster while the Pahang population was on its own.

[error in script]

Item Type:	Article
AGROVOC Term:	Tor tambroides
AGROVOC Term:	Mystus nemurus
AGROVOC Term:	Freshwater catfish
AGROVOC Term:	Microsatellites
AGROVOC Term:	Data analysis
AGROVOC Term:	DNA
AGROVOC Term:	Genetic distance
AGROVOC Term:	Alleles
AGROVOC Term:	Heterozygotes
AGROVOC Term:	Species
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	27 Apr 2025 23:32
URI:	http://webagris.upm.edu.my/id/eprint/22779

Actions (login required)

View Item