Citation
Othman Omar, . and Siti Nor Akmar Abdullah, . and Syaiful Bahri Panjaitan, . and Maheran Abdul Aziz, . and Sariah Meon, . Somatic embryogenesis from scutellar embryo of Oryza sativa L. var. MR219. pp. 185-194. ISSN 1511-3701
Abstract
Somatic embryogenesis is an efficient plant regeneration system and it is a potentially useful tool for genetic transformation. An experiment was carried out on somatic embryogenesis from scutellar embryo of rice var. MR219. High intensity of callus formation (100) was initiated through culturing the scutellar embryo on modified MS medium with the macro nutrients reduced to half-strength and supplemented with different 2 4-D concentrations (1 2 4 and 6 mgL-1). Meanwhile the highest percentage of embryogenic callus formation (80) was obtained on the modified MS medium containing 4 mgL-1 2 4-D. The calli produced were yellowish and friable with nodular structures on the surface. Rounded cells with highly dense cytoplasm were also observed under an inverted microscope and their viability was confirmed based on the apple green fluorescence staining in the fluorescein diacetate (FDA) solution. High mean number of somatic embryos was also produced in this treatment at 85 somatic embryos per explant. Upon transferring the somatic embryos onto the modified MS medium with 2 mgL-1 BAP and 0.05 mgL-1 NAA for germination 82.5 of the somatic embryos were germinated into the seedlings.
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Abstract
Somatic embryogenesis is an efficient plant regeneration system and it is a potentially useful tool for genetic transformation. An experiment was carried out on somatic embryogenesis from scutellar embryo of rice var. MR219. High intensity of callus formation (100) was initiated through culturing the scutellar embryo on modified MS medium with the macro nutrients reduced to half-strength and supplemented with different 2 4-D concentrations (1 2 4 and 6 mgL-1). Meanwhile the highest percentage of embryogenic callus formation (80) was obtained on the modified MS medium containing 4 mgL-1 2 4-D. The calli produced were yellowish and friable with nodular structures on the surface. Rounded cells with highly dense cytoplasm were also observed under an inverted microscope and their viability was confirmed based on the apple green fluorescence staining in the fluorescein diacetate (FDA) solution. High mean number of somatic embryos was also produced in this treatment at 85 somatic embryos per explant. Upon transferring the somatic embryos onto the modified MS medium with 2 mgL-1 BAP and 0.05 mgL-1 NAA for germination 82.5 of the somatic embryos were germinated into the seedlings.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Rice |
| AGROVOC Term: | Oryza sativa |
| AGROVOC Term: | Somatic embryos |
| AGROVOC Term: | Embryogenesis |
| AGROVOC Term: | Tissue culture |
| AGROVOC Term: | 2 4-dichlorophenoxyacetic acid |
| AGROVOC Term: | Benzylaminopurine |
| AGROVOC Term: | Germination |
| AGROVOC Term: | Seedlings |
| AGROVOC Term: | Callus |
| Geographical Term: | Malaysia |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 28 Apr 2025 00:10 |
| URI: | http://webagris.upm.edu.my/id/eprint/22789 |
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