Mutation breeding of Phaseolus vulgaris L.: studies on the effects of irradiation dosage to resolve a suitable procedure of handling M and M generations

Cheah C. H., . and Lim E. S., . Mutation breeding of Phaseolus vulgaris L.: studies on the effects of irradiation dosage to resolve a suitable procedure of handling M and M generations. pp. 184-191. ISSN 0126-6128

Seedling growth studies on gamma-irradiated seeds of Phaseolus vulgaris were conducted in the greenhouse and the field. No significant differences in germination scores between the unirradiated control and the seeds exposed to 10 20 25 30 35 and 40 Krads of gamma radiation were obtained. Exposure to 100 Krads however was lethal to the apical meristem. Growth of the epicotyl under greenhouse conditions was the most affected by gamma radiation. Based upon a 30 reduction in epicotyl length the optimum dose for irradiation was 30 Krads. At this level of irradiation the survival score at harvest under field conditions was 50 of that of the unirradiated control. Treatment with 30 Krads also gave the highest number of solid chlorophyll mutants. While not all the chlorophyll mutants were scored in the Bs (si gle seed bulk) populations the occurrence was consistently higher than that for the corresponding Bp (single pod bulk) populations. Based on these results it is recommended that : a) the most suitable level of gamma radiation to use in a mutation breeding programme is 30 Krads; b) the Mx seeds should be field-planted at double the planting density of the control; c) the Mx plants should be harvested such that the Bs (single seed bulk) population be used for screening of the mutants.

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Abstract

Seedling growth studies on gamma-irradiated seeds of Phaseolus vulgaris were conducted in the greenhouse and the field. No significant differences in germination scores between the unirradiated control and the seeds exposed to 10 20 25 30 35 and 40 Krads of gamma radiation were obtained. Exposure to 100 Krads however was lethal to the apical meristem. Growth of the epicotyl under greenhouse conditions was the most affected by gamma radiation. Based upon a 30 reduction in epicotyl length the optimum dose for irradiation was 30 Krads. At this level of irradiation the survival score at harvest under field conditions was 50 of that of the unirradiated control. Treatment with 30 Krads also gave the highest number of solid chlorophyll mutants. While not all the chlorophyll mutants were scored in the Bs (si gle seed bulk) populations the occurrence was consistently higher than that for the corresponding Bp (single pod bulk) populations. Based on these results it is recommended that : a) the most suitable level of gamma radiation to use in a mutation breeding programme is 30 Krads; b) the Mx seeds should be field-planted at double the planting density of the control; c) the Mx plants should be harvested such that the Bs (single seed bulk) population be used for screening of the mutants.

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Item Type:	Article
AGROVOC Term:	Phaseolus vulgaris
AGROVOC Term:	Mutation breeding
AGROVOC Term:	Irradiation
AGROVOC Term:	Seedlings
AGROVOC Term:	Apical meristems
AGROVOC Term:	Epicotyls
AGROVOC Term:	Greenhouses
AGROVOC Term:	Gamma radiation
AGROVOC Term:	Survival
AGROVOC Term:	Harvesters
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	28 Apr 2025 00:26
URI:	http://webagris.upm.edu.my/id/eprint/22862

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