Citation
Arokiaraj P., . and Zainorlina Mohd Zainuddin, . and Siti Zakiah Zailani, . and Wan K. L., . and Mohd Nor Mat Isa, . and Chow K. S., . and Azlina Bahari, . and Halimah Alias, . and Nasrul Hadi Shaffe, . Characterisation of type 2 and 3 metallothioneins from Hevea brasiliensis bark. pp. 257-270. ISSN 1511-1768
Abstract
A small collection of ESTs was generated from the bark of the rubber tree clone RRIM 2025. Low molecular weight metal-binding proteins metallothioneins (MTs) were found to be very abundant in the bark gene expression profile. Multiple sequence alignment and phylogenetic analysis between three Hevea MTs and other plant MTs identified them as type 2 and type 3 proteins. Semi-quantitative PCR analysis indicated that each MT was differentially expressed between bark leaves and latex with the lowest transcript abundance in latex. In latex from ethephon-treated trees all MT isoforms were induced at four hours after treatment while only one of them showed decreased expression after 24 hours. Our findings indicate the existence of a multiple gene family for Hevea MTs and varying roles played by isoforms derived from different rubber tree tissues.
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Abstract
A small collection of ESTs was generated from the bark of the rubber tree clone RRIM 2025. Low molecular weight metal-binding proteins metallothioneins (MTs) were found to be very abundant in the bark gene expression profile. Multiple sequence alignment and phylogenetic analysis between three Hevea MTs and other plant MTs identified them as type 2 and type 3 proteins. Semi-quantitative PCR analysis indicated that each MT was differentially expressed between bark leaves and latex with the lowest transcript abundance in latex. In latex from ethephon-treated trees all MT isoforms were induced at four hours after treatment while only one of them showed decreased expression after 24 hours. Our findings indicate the existence of a multiple gene family for Hevea MTs and varying roles played by isoforms derived from different rubber tree tissues.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Hevea brasiliensis |
| AGROVOC Term: | Metallothionein |
| AGROVOC Term: | Bark |
| AGROVOC Term: | Molecular weight |
| AGROVOC Term: | Clones |
| AGROVOC Term: | Gene expression |
| AGROVOC Term: | Leaves |
| AGROVOC Term: | Latex |
| AGROVOC Term: | Ethephon |
| AGROVOC Term: | Genetic diversity within species |
| Geographical Term: | Malaysia |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 28 Apr 2025 02:30 |
| URI: | http://webagris.upm.edu.my/id/eprint/22986 |
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