Molecular characterisation of the sucrose transporter HbSUT1B in relation to rubber production in latex cells

Chrestin H., . and Franchel J., . and Sakr S., . and Dusotoit-Coucaud A., . and Granet F., . and Brunel N., . Molecular characterisation of the sucrose transporter HbSUT1B in relation to rubber production in latex cells. pp. 125-139. ISSN 1511-1768

The rubber synthesis in latex cells requires sucrose. These particular cells are heterotrophic ones and are apoplastically connected to other tissues. Such features imply that the rubber yield may depend on the latex cell efficiency to absorb and to use the imported sucrose. This study investigates the sucrose transporters as key genes for rubber biosynthesis involved in the ethylene-stimulated rubber yield. Seven putative sucrose transporters had been isolated from a latex-derived cDNA library of PB 217. Expression profiling of these sucrose transporters under ethylene stimulation in PB 217 underlined the role of one sucrose transporter: HbSUT1B ( Hevea brasiliensis Sucrose Transporter 6) (Dusotoit-Coucaud et al. 2007). This paper reports the next stage in HbSUT1B molecular characterisation. Its expression in different parts of the rubber tree was studied. It was preferentially expressed in leaves and bark. This result was completed by in-situ hybridisation experiments. In addition promoter cloning and analysis revealed some clues for the wounding regulation of HbSUT1B. The HbSUT1B expression was analysed in response to different treatments in latex or bark tissues. HbSUT1B was considerably up-regulated by hormones and wounding in bark but not in latex. This regulation appeared to be tissue-specific.

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Abstract

The rubber synthesis in latex cells requires sucrose. These particular cells are heterotrophic ones and are apoplastically connected to other tissues. Such features imply that the rubber yield may depend on the latex cell efficiency to absorb and to use the imported sucrose. This study investigates the sucrose transporters as key genes for rubber biosynthesis involved in the ethylene-stimulated rubber yield. Seven putative sucrose transporters had been isolated from a latex-derived cDNA library of PB 217. Expression profiling of these sucrose transporters under ethylene stimulation in PB 217 underlined the role of one sucrose transporter: HbSUT1B ( Hevea brasiliensis Sucrose Transporter 6) (Dusotoit-Coucaud et al. 2007). This paper reports the next stage in HbSUT1B molecular characterisation. Its expression in different parts of the rubber tree was studied. It was preferentially expressed in leaves and bark. This result was completed by in-situ hybridisation experiments. In addition promoter cloning and analysis revealed some clues for the wounding regulation of HbSUT1B. The HbSUT1B expression was analysed in response to different treatments in latex or bark tissues. HbSUT1B was considerably up-regulated by hormones and wounding in bark but not in latex. This regulation appeared to be tissue-specific.

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Item Type:	Article
AGROVOC Term:	Rubber
AGROVOC Term:	Latex
AGROVOC Term:	Sucrose
AGROVOC Term:	Biosynthesis
AGROVOC Term:	Ethylene
AGROVOC Term:	Rubber industry
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/23050

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