An efficient method for the isolation of good quality RNA from bark tissues of mature rubber trees.


Citation

Jacob J., . and Nataraja K.N., . and Udayakumar M., . and Mohamed Sathik M.B., . and Thomas M., . An efficient method for the isolation of good quality RNA from bark tissues of mature rubber trees. pp. 182-189. ISSN 1511-1768

Abstract

In this study an attempt has been made to extract good quality RNA from soft bark tissues of Hevea which generally contain plenty of interfering compounds such as phenolic compounds tannins lignins and carbohydrates. The method explained here involves removal of phenolic compounds using polyvinyl pyrrolidone and selective precipitation of RNA using lithium chloride. The purity of RNA prepared by this method was tested for its ability to get a good quality cDNA prepared. The cDNA produced from total RNA preparation was further confirmed by amplification of specific house keeping genes using quantitative real time PCR method. This protocol appears best suited for Hevea bark that is rich in several contaminating factors.


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Abstract

In this study an attempt has been made to extract good quality RNA from soft bark tissues of Hevea which generally contain plenty of interfering compounds such as phenolic compounds tannins lignins and carbohydrates. The method explained here involves removal of phenolic compounds using polyvinyl pyrrolidone and selective precipitation of RNA using lithium chloride. The purity of RNA prepared by this method was tested for its ability to get a good quality cDNA prepared. The cDNA produced from total RNA preparation was further confirmed by amplification of specific house keeping genes using quantitative real time PCR method. This protocol appears best suited for Hevea bark that is rich in several contaminating factors.

Additional Metadata

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Item Type: Article
AGROVOC Term: Isolation
AGROVOC Term: Bark
AGROVOC Term: Rubber
AGROVOC Term: Hevea brasiliensis
AGROVOC Term: Tapping
AGROVOC Term: Rubber crops
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 06:28
URI: http://webagris.upm.edu.my/id/eprint/23117

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