Correlation between total extractable protein and antigen contents with allergen content of NR gloves and chemical interference on protein assays.

Ruhida A. R., . and Nurul Hayati Y., . and Amir Hashim M. Y., . and Hasma H., . and Lau C. H., . Correlation between total extractable protein and antigen contents with allergen content of NR gloves and chemical interference on protein assays. pp. 56-70. ISSN 1511-1768

The allergen or allergenic protein (AgP) content of NR latex examination gloves as determined by the IgE-ELISA inhibition method was compared with the total extractable protein (EP) and the antigen or antigenic protein (AP) contents of the same gloves. Results showed poor linear correlation between AgP with EP andAP contents. It was however observed that low (10AU/mL) to moderate (10 AU/mL - 100 AU/mL) allergen values corresponded to EP(MS 1392:1998) 100 fjg/g EP(ASTM D5712-95) 60 /jg/g EP(ASTM D5712-99) 60 /jg/dm EP(EN 455- 3:1999) 60 /jg/g andAP (ASTM D6499-00) 10 /jg/dm. Above these EP limits the allergen levels were high ( 100 AU/mL). Comparison between EP(ASTM 99) and AP showed a high probability of gloves with EP values of 60 jg/dm contained AP below the recommended limit of 10 pg/dm although there were still a significant proportion of gloves with EP values of 60 pg/dm - 100 pg/dm meeting the AP limit. Above this the AP content exceeded the limit. Interference on AgP or AP determination could be expected when gloves with EP 60 pg/dm contained AgP 100 AU/mL or AP 10 pg/dm. Likewise interference on EP determination could occur when gloves with EP 60 pg/dm contained AgP 100 AU/mL orAP 10 pg/dm. Although EP determination with correction could check the interference on total EP assay the interference on allergen and antigen assays could only be ascertained via carrying out the EP assay as well.

Full text available from: Official URL: http://vitaldoc.lgm.gov.my:8060/vital/access/servi...

Abstract

The allergen or allergenic protein (AgP) content of NR latex examination gloves as determined by the IgE-ELISA inhibition method was compared with the total extractable protein (EP) and the antigen or antigenic protein (AP) contents of the same gloves. Results showed poor linear correlation between AgP with EP andAP contents. It was however observed that low (10AU/mL) to moderate (10 AU/mL - 100 AU/mL) allergen values corresponded to EP(MS 1392:1998) 100 fjg/g EP(ASTM D5712-95) 60 /jg/g EP(ASTM D5712-99) 60 /jg/dm EP(EN 455- 3:1999) 60 /jg/g andAP (ASTM D6499-00) 10 /jg/dm. Above these EP limits the allergen levels were high ( 100 AU/mL). Comparison between EP(ASTM 99) and AP showed a high probability of gloves with EP values of 60 jg/dm contained AP below the recommended limit of 10 pg/dm although there were still a significant proportion of gloves with EP values of 60 pg/dm - 100 pg/dm meeting the AP limit. Above this the AP content exceeded the limit. Interference on AgP or AP determination could be expected when gloves with EP 60 pg/dm contained AgP 100 AU/mL or AP 10 pg/dm. Likewise interference on EP determination could occur when gloves with EP 60 pg/dm contained AgP 100 AU/mL orAP 10 pg/dm. Although EP determination with correction could check the interference on total EP assay the interference on allergen and antigen assays could only be ascertained via carrying out the EP assay as well.

[error in script]

Item Type:	Article
AGROVOC Term:	Proteins
AGROVOC Term:	Antigens
AGROVOC Term:	Allergens
AGROVOC Term:	Chemical reactions
AGROVOC Term:	Natural rubber
AGROVOC Term:	Standards
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	28 Apr 2025 03:18
URI:	http://webagris.upm.edu.my/id/eprint/23126

Actions (login required)

View Item