Differentiating races of Corynespora cassiicola using RAPD and internal transcribed spacer markers

Safiah Atan, . and Noor Hisham Hamid, . Differentiating races of Corynespora cassiicola using RAPD and internal transcribed spacer markers. pp. 58-64. ISSN 1511-1768

Nine isolates of corynespora cassiicola from Hevea brasiliensis were analysed using two molecular marker techniques: random amplified polymorphic DNA (RAPD); and the amplification of the internal transcribed spacer (ITS) regions of ribosomal DNA. RAPD distinguished two groups of isolates i.e. isolates infectious to RRIM 2020 clones and isolates that infected RRIM 600 and several other clones of H. brasiliensis. The ITS region of eight out of nine isolates were found to be monomorphic.

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Abstract

Nine isolates of corynespora cassiicola from Hevea brasiliensis were analysed using two molecular marker techniques: random amplified polymorphic DNA (RAPD); and the amplification of the internal transcribed spacer (ITS) regions of ribosomal DNA. RAPD distinguished two groups of isolates i.e. isolates infectious to RRIM 2020 clones and isolates that infected RRIM 600 and several other clones of H. brasiliensis. The ITS region of eight out of nine isolates were found to be monomorphic.

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Item Type:	Article
AGROVOC Term:	Hevea brasiliensis
AGROVOC Term:	Corynespora cassiicola
AGROVOC Term:	Molecular markers
AGROVOC Term:	Isolation techniques
AGROVOC Term:	Cloning
AGROVOC Term:	Random amplified polymorphic DNA
AGROVOC Term:	Infectious diseases
AGROVOC Term:	Differential diagnosis
AGROVOC Term:	Plant disease control
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	28 Apr 2025 03:23
URI:	http://webagris.upm.edu.my/id/eprint/23149

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