Citation
Rosnina Yusoff, . and Abdul Wahid Haron, . and Degu Nurhusien Yimer, . and H. Salman, . and Falah Hasan Baiee, . and A. A. Tarig, . and Mohamed Ariff Omar, . and Mada Khumran A., . Hypo-osmotic swelling test modification to enhance cell membrane integrity evaluation in cryopreserved bull semen. pp. 257-268. ISSN ISSN2231-8542(Online).
Abstract
The objective of this study was to enhance the hypo-osmotic swelling test evaluation when it reads under light microscope using 5 formaldehyde-fixed sperm solution buffer (FSSB). Twenty four ejaculates were collected from six crossbred bulls using electro-ejaculator (EE). Tris-egg yolk extender was used to cryopreserve the semen. Concentration volume motility morphology and viability rates of fresh semen were evaluated and samples were cryopreserved in liquid nitrogen. After two weeks of liquid nitrogen treatment (freezing) the motility morphology and viability rate of the semen were evaluated. In order to carry out a hypo-osmotic swelling test post-thaw semen was divided into four aliquots based on period of incubation (30 or 60 minutes) adding FSSB to half the samples. The components of FSSB were 5 formaldehyde and 1 eosin-nigrosin stain in PBS. Results showed that F60 (61.48 0.89) resulted in higher percentage (P0.05) with N60 (60.900.70). In conclusion adding 10 l of FSSB after 60 minute of incubation with hypo-osmotic swelling solution (HOSS) will enhance evaluation of the hypo-osmotic swelling test (HOST) under light microscope.
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Abstract
The objective of this study was to enhance the hypo-osmotic swelling test evaluation when it reads under light microscope using 5 formaldehyde-fixed sperm solution buffer (FSSB). Twenty four ejaculates were collected from six crossbred bulls using electro-ejaculator (EE). Tris-egg yolk extender was used to cryopreserve the semen. Concentration volume motility morphology and viability rates of fresh semen were evaluated and samples were cryopreserved in liquid nitrogen. After two weeks of liquid nitrogen treatment (freezing) the motility morphology and viability rate of the semen were evaluated. In order to carry out a hypo-osmotic swelling test post-thaw semen was divided into four aliquots based on period of incubation (30 or 60 minutes) adding FSSB to half the samples. The components of FSSB were 5 formaldehyde and 1 eosin-nigrosin stain in PBS. Results showed that F60 (61.48 0.89) resulted in higher percentage (P0.05) with N60 (60.900.70). In conclusion adding 10 l of FSSB after 60 minute of incubation with hypo-osmotic swelling solution (HOSS) will enhance evaluation of the hypo-osmotic swelling test (HOST) under light microscope.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Bulls |
| AGROVOC Term: | Cell membranes |
| AGROVOC Term: | Cryopreservation |
| AGROVOC Term: | Semen |
| AGROVOC Term: | Motility |
| AGROVOC Term: | morphology |
| AGROVOC Term: | Viability |
| AGROVOC Term: | Liquid nitrogen |
| AGROVOC Term: | Swelling |
| AGROVOC Term: | Egg incubation |
| Geographical Term: | Malaysia |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 28 Apr 2025 03:29 |
| URI: | http://webagris.upm.edu.my/id/eprint/23239 |
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