A rapid assay for the incorporation of isopentenyl disphosphate in rubber biosynthesis


Citation

Faridah Yusof, . and Audley B. G., . and Fyaz Ismail, . and Walker J. M., . A rapid assay for the incorporation of isopentenyl disphosphate in rubber biosynthesis. pp. 48-66. ISSN 1511-1768

Abstract

A rapid sensitive assay for comparing relative rates of rubber biosynthesis in vitro is presented. This method is considerably faster than those previously described. Due to its simplicity the assay is appropriate for monitoring the purification of factors involved in rubber biosynthesis. However it is necessary to be wary of the possible effects of certain ions such as Mg2 Na and phosphates which at certain concentrations considerably affect the rate of rubber biosynthesis.


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Abstract

A rapid sensitive assay for comparing relative rates of rubber biosynthesis in vitro is presented. This method is considerably faster than those previously described. Due to its simplicity the assay is appropriate for monitoring the purification of factors involved in rubber biosynthesis. However it is necessary to be wary of the possible effects of certain ions such as Mg2 Na and phosphates which at certain concentrations considerably affect the rate of rubber biosynthesis.

Additional Metadata

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Item Type: Article
AGROVOC Term: Rubber
AGROVOC Term: Hevea brasiliensis
AGROVOC Term: Biosynthesis
AGROVOC Term: In vitro
AGROVOC Term: Purification
AGROVOC Term: Centrifugation
AGROVOC Term: Isoprenoids
AGROVOC Term: Thin layer chromatography
AGROVOC Term: Analytical methods
AGROVOC Term: Enzymes
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 06:28
URI: http://webagris.upm.edu.my/id/eprint/23312

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