Citation
Coupe M., . and Breton F., . and Sanier C., . and D’Auzac J., . Demonstration of B-1 3-glucanase activities in lutoids of Hevea brasiliensis latex. pp. 37-45. ISSN 0127-7065
Abstract
b-l 3-glucanase activity generally known to be involved in plant defence reactions against pathogens was observed in extracts of lutoids from Hevea latex. The b-1 3-glucanase activities detected after native isoelectrofocusing (IEF) led to the visualisation of five bands consisting of three basic isozymes (pI 10.00 0.02; 9.32 0.24 and 9.0 0.22) and two acid isozymes (pI 5.49 0.02 and 4.88 0.03). Immunodetection with tobacco polyclonal antibodies against acid and basic b-l 3-glucanases revealed the same protein profile characterised by three major bands with molecular weights of 38.9 1.2 36.4 0.89 and 30.1 1 kD respectively. The presence of apparently constitutive chitinases and b-1 3-glucanases in lutoids is discussed.
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Abstract
b-l 3-glucanase activity generally known to be involved in plant defence reactions against pathogens was observed in extracts of lutoids from Hevea latex. The b-1 3-glucanase activities detected after native isoelectrofocusing (IEF) led to the visualisation of five bands consisting of three basic isozymes (pI 10.00 0.02; 9.32 0.24 and 9.0 0.22) and two acid isozymes (pI 5.49 0.02 and 4.88 0.03). Immunodetection with tobacco polyclonal antibodies against acid and basic b-l 3-glucanases revealed the same protein profile characterised by three major bands with molecular weights of 38.9 1.2 36.4 0.89 and 30.1 1 kD respectively. The presence of apparently constitutive chitinases and b-1 3-glucanases in lutoids is discussed.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Hevea brasiliensis |
| AGROVOC Term: | Beta glucanase |
| AGROVOC Term: | Latex |
| AGROVOC Term: | Pathogens |
| AGROVOC Term: | Defence mechanisms |
| AGROVOC Term: | Isozymes |
| AGROVOC Term: | Polyclonal antibodies |
| AGROVOC Term: | Molecular weight |
| AGROVOC Term: | Cytoplasm |
| AGROVOC Term: | Enzymatic activity |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:28 |
| URI: | http://webagris.upm.edu.my/id/eprint/23380 |
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